Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

Marco Rinaudo; Claudio Grassi; Sara Cocco; Massimo Gennarelli; Chiara Magri; Erika Vitali; Edoardo Giacopuzzi; Paolo Martini; Alessandro Barbon

doi:10.3390/ijms22147629

Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

Marco Rinaudo, Claudio Grassi, Sara Cocco, Massimo Gennarelli, Chiara Magri, Erika Vitali, Edoardo Giacopuzzi, Paolo Martini, Alessandro Barbon

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

Abstract

The 3xTg-AD mouse is a widely used model in the study of Alzheimer’s Disease (AD). It has been extensively characterized from both the anatomical and behavioral point of view, but poorly studied at the transcriptomic level. For the first time, we characterize the whole blood transcriptome of the 3xTg-AD mouse at three and six months of age and evaluate how its gene expression is modulated by transcranial direct current stimulation (tDCS). RNA-seq analysis revealed 183 differentially expressed genes (DEGs) that represent a direct signature of the genetic background of the mouse. Moreover, in the 6-month-old 3xTg-AD mice, we observed a high number of DEGs that could represent good peripheral biomarkers of AD symptomatology onset. Finally, tDCS was associated with gene expression changes in the 3xTg-AD, but not in the control mice. In conclusion, this study provides an in-depth molecular characterization of the 3xTg-AD mouse and suggests that blood gene expression can be used to identify new biomarkers of AD progression and treatment effects.

Lingua originale	English
pagine (da-a)	N/A-N/A
Numero di pagine	21
Rivista	International Journal of Molecular Sciences
Volume	22
DOI	https://doi.org/10.3390/ijms22147629
Stato di pubblicazione	Pubblicato - 2021

Keywords

3xTg-AD mouse
Alzheimer Disease
Alzheimer’s disease
Amyloid beta-Peptides
Amyloid beta-Protein Precursor
Animals
Blood Cells
Disease Models, Animal
Gene Expression Profiling
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
RNA-Seq
Transcranial Direct Current Stimulation
Transcriptome
Whole Exome Sequencing
tau Proteins
transcranial direct current stimulation

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Rinaudo, Marco ; Grassi, Claudio ; Cocco, Sara ; Gennarelli, Massimo ; Magri, Chiara ; Vitali, Erika ; Giacopuzzi, Edoardo ; Martini, Paolo ; Barbon, Alessandro. / Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS). In: International Journal of Molecular Sciences. 2021 ; Vol. 22. pagg. N/A-N/A.

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author = "Marco Rinaudo and Claudio Grassi and Sara Cocco and Massimo Gennarelli and Chiara Magri and Erika Vitali and Edoardo Giacopuzzi and Paolo Martini and Alessandro Barbon",

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Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS). / Rinaudo, Marco ; Grassi, Claudio; Cocco, Sara; Gennarelli, Massimo; Magri, Chiara; Vitali, Erika; Giacopuzzi, Edoardo; Martini, Paolo; Barbon, Alessandro.

In: International Journal of Molecular Sciences, Vol. 22, 2021, pag. N/A-N/A.

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

TY - JOUR

T1 - Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

AU - Rinaudo, Marco

AU - Grassi, Claudio

AU - Cocco, Sara

AU - Gennarelli, Massimo

AU - Magri, Chiara

AU - Vitali, Erika

AU - Giacopuzzi, Edoardo

AU - Martini, Paolo

AU - Barbon, Alessandro

PY - 2021

Y1 - 2021

N2 - The 3xTg-AD mouse is a widely used model in the study of Alzheimer’s Disease (AD). It has been extensively characterized from both the anatomical and behavioral point of view, but poorly studied at the transcriptomic level. For the first time, we characterize the whole blood transcriptome of the 3xTg-AD mouse at three and six months of age and evaluate how its gene expression is modulated by transcranial direct current stimulation (tDCS). RNA-seq analysis revealed 183 differentially expressed genes (DEGs) that represent a direct signature of the genetic background of the mouse. Moreover, in the 6-month-old 3xTg-AD mice, we observed a high number of DEGs that could represent good peripheral biomarkers of AD symptomatology onset. Finally, tDCS was associated with gene expression changes in the 3xTg-AD, but not in the control mice. In conclusion, this study provides an in-depth molecular characterization of the 3xTg-AD mouse and suggests that blood gene expression can be used to identify new biomarkers of AD progression and treatment effects.

AB - The 3xTg-AD mouse is a widely used model in the study of Alzheimer’s Disease (AD). It has been extensively characterized from both the anatomical and behavioral point of view, but poorly studied at the transcriptomic level. For the first time, we characterize the whole blood transcriptome of the 3xTg-AD mouse at three and six months of age and evaluate how its gene expression is modulated by transcranial direct current stimulation (tDCS). RNA-seq analysis revealed 183 differentially expressed genes (DEGs) that represent a direct signature of the genetic background of the mouse. Moreover, in the 6-month-old 3xTg-AD mice, we observed a high number of DEGs that could represent good peripheral biomarkers of AD symptomatology onset. Finally, tDCS was associated with gene expression changes in the 3xTg-AD, but not in the control mice. In conclusion, this study provides an in-depth molecular characterization of the 3xTg-AD mouse and suggests that blood gene expression can be used to identify new biomarkers of AD progression and treatment effects.

KW - 3xTg-AD mouse

KW - Alzheimer Disease

KW - Alzheimer’s disease

KW - Amyloid beta-Peptides

KW - Amyloid beta-Protein Precursor

KW - Animals

KW - Blood Cells

KW - Disease Models, Animal

KW - Gene Expression Profiling

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - RNA-Seq

KW - Transcranial Direct Current Stimulation

KW - Transcriptome

KW - Whole Exome Sequencing

KW - tau Proteins

KW - transcranial direct current stimulation

KW - 3xTg-AD mouse

KW - Alzheimer Disease

KW - Alzheimer’s disease

KW - Amyloid beta-Peptides

KW - Amyloid beta-Protein Precursor

KW - Animals

KW - Blood Cells

KW - Disease Models, Animal

KW - Gene Expression Profiling

KW - Male

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - RNA-Seq

KW - Transcranial Direct Current Stimulation

KW - Transcriptome

KW - Whole Exome Sequencing

KW - tau Proteins

KW - transcranial direct current stimulation

UR - http://hdl.handle.net/10807/184729

U2 - 10.3390/ijms22147629

DO - 10.3390/ijms22147629

M3 - Article

VL - 22

SP - N/A-N/A

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

ER -

Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

Abstract

Keywords

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