Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)

Marco Rinaudo, Claudio Grassi, Sara Cocco, Massimo Gennarelli, Chiara Magri, Erika Vitali, Edoardo Giacopuzzi, Paolo Martini, Alessandro Barbon

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

The 3xTg-AD mouse is a widely used model in the study of Alzheimer’s Disease (AD). It has been extensively characterized from both the anatomical and behavioral point of view, but poorly studied at the transcriptomic level. For the first time, we characterize the whole blood transcriptome of the 3xTg-AD mouse at three and six months of age and evaluate how its gene expression is modulated by transcranial direct current stimulation (tDCS). RNA-seq analysis revealed 183 differentially expressed genes (DEGs) that represent a direct signature of the genetic background of the mouse. Moreover, in the 6-month-old 3xTg-AD mice, we observed a high number of DEGs that could represent good peripheral biomarkers of AD symptomatology onset. Finally, tDCS was associated with gene expression changes in the 3xTg-AD, but not in the control mice. In conclusion, this study provides an in-depth molecular characterization of the 3xTg-AD mouse and suggests that blood gene expression can be used to identify new biomarkers of AD progression and treatment effects.
Lingua originaleEnglish
pagine (da-a)N/A-N/A
Numero di pagine21
RivistaInternational Journal of Molecular Sciences
Volume22
DOI
Stato di pubblicazionePubblicato - 2021

Keywords

  • 3xTg-AD mouse
  • Alzheimer Disease
  • Alzheimer’s disease
  • Amyloid beta-Peptides
  • Amyloid beta-Protein Precursor
  • Animals
  • Blood Cells
  • Disease Models, Animal
  • Gene Expression Profiling
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • RNA-Seq
  • Transcranial Direct Current Stimulation
  • Transcriptome
  • Whole Exome Sequencing
  • tau Proteins
  • transcranial direct current stimulation

Fingerprint

Entra nei temi di ricerca di 'Whole Blood Transcriptome Characterization of 3xTg-AD Mouse and Its Modulation by Transcranial Direct Current Stimulation (tDCS)'. Insieme formano una fingerprint unica.

Cita questo