TY - JOUR
T1 - Variability and Number of Circulating Complementary Sex Determiner (Csd) Alleles in a Breeding Population of Italian Honeybees under Controlled Mating
AU - De Iorio, Maria Grazia
AU - Lazzari, Barbara
AU - Colli, Licia
AU - Pagnacco, Giulio
AU - Minozzi, Giulietta
PY - 2024
Y1 - 2024
N2 - In Apis mellifera, csd is the primary gene involved in sex determination: haploid hemizygous eggs develop as drones, while females develop from eggs heterozygous for the csd gene. If diploid eggs are homozygous for the csd gene, diploid drones will develop, but will be eaten by worker bees before they are born. Therefore, high csd allelic diversity is a priority for colony survival and breeding. This study aims to investigate the variability of the hypervariable region (HVR) of the csd gene in bees sampled in an apiary under a selection scheme. To this end, an existing dataset of 100 whole-genome sequences was analyzed with a validated pipeline based on de novo assembly of sequences within the HVR region. In total, 102 allelic sequences were reconstructed and translated into amino acid sequences. Among these, 47 different alleles were identified, 44 of which had previously been observed, while 3 are novel alleles. The results show a high variability in the csd region in this breeding population of honeybees.
AB - In Apis mellifera, csd is the primary gene involved in sex determination: haploid hemizygous eggs develop as drones, while females develop from eggs heterozygous for the csd gene. If diploid eggs are homozygous for the csd gene, diploid drones will develop, but will be eaten by worker bees before they are born. Therefore, high csd allelic diversity is a priority for colony survival and breeding. This study aims to investigate the variability of the hypervariable region (HVR) of the csd gene in bees sampled in an apiary under a selection scheme. To this end, an existing dataset of 100 whole-genome sequences was analyzed with a validated pipeline based on de novo assembly of sequences within the HVR region. In total, 102 allelic sequences were reconstructed and translated into amino acid sequences. Among these, 47 different alleles were identified, 44 of which had previously been observed, while 3 are novel alleles. The results show a high variability in the csd region in this breeding population of honeybees.
KW - alleles variability
KW - Apis mellifera
KW - hypervariable region
KW - complementary sex determiner gene
KW - breeding population
KW - alleles variability
KW - Apis mellifera
KW - hypervariable region
KW - complementary sex determiner gene
KW - breeding population
UR - http://hdl.handle.net/10807/300829
U2 - 10.3390/genes15060652
DO - 10.3390/genes15060652
M3 - Article
SN - 2073-4425
VL - 15
SP - 1
EP - 10
JO - Genes
JF - Genes
ER -