Validation of antibiotics in catfish by on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry

Luana Maggi, Jorge Hurtado De Mendoza, Liliana Bonetto, Beatriz Rodríguez Carmena, Alicia Lezana, Francisco A. Mocholí, Manuel Carmona

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

23 Citazioni (Scopus)

Abstract

For the first time automated on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of 13 antibiotics (sulfonamides and tetracyclines) in catfish. The method proposed was validated according to Commission Decision 2002/657/EC, showing good linearity between 2 and 350 µg kg-1, high recovery (80–99%) and reproducibility (13–20%) values, lower detection limits than 0.1 µg kg-1, and quantification limits under 2.4 µg kg-1 (between 39 and 84 times lower than the MRL fixed by the EU). Moreover, the proposed method was also used to determine sulfonamides and tetracyclines in 16 out of 107 samples, all previously analysed by microbiological screening that gave positive results. Five out of 13 antibiotics were found, having tetracycline the higher occurrence (10 samples); in all cases the concentrations were lower than the MRL established.
Lingua originaleEnglish
pagine (da-a)1149-1155
Numero di pagine7
RivistaFood Chemistry
Volume134
DOI
Stato di pubblicazionePubblicato - 2012
Pubblicato esternamenteYes

Keywords

  • Antibiotics
  • Catfish
  • Residues
  • XLC–MS/MS

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