Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B-1 Carry Over in Milk

Luciano Polonelli; Laura Giovati; Walter Magliani; Stefania Conti; Stefano Sforza; Alessandro Calabretta; Claudio Casoli; Paola Ronzi; Ester Grilli; Antonio Gallo; Francesco Masoero; Gianfranco Piva

doi:10.1371/journal.pone.0026777

Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B-1 Carry Over in Milk

Luciano Polonelli, Laura Giovati, Walter Magliani, Stefania Conti, Stefano Sforza, Alessandro Calabretta, Claudio Casoli, Paola Ronzi, Ester Grilli, Antonio Gallo, Francesco Masoero, Gianfranco Piva

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

18 Citazioni (Scopus)

Abstract

The potential of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB1-KLH) in controlling the carry over of the aflatoxin B1 (AFB1) metabolite aflatoxin M1 (AFM1) in cow milk is reported. AFB1 is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB1 is AFB1 chemically modified as AFB1-1(Ocarboxymethyl) oxime. In comparison to AFB1, AnAFB1 has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB1-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB1 IgG antibodies (Abs) which were cross reactive with AFB1, AFG1, and AFG2. The elicited anti- AFB1 Abs were able to hinder the secretion of AFM1 into the milk of cows continuously fed with AFB1. Vaccination of lactating animals with conjugated AnAFB1 may represent a solution to the public hazard constituted by milk and cheese contaminated

Lingua originale	English
pagine (da-a)	1-9
Numero di pagine	9
Rivista	PLoS One
Volume	6
DOI	https://doi.org/10.1371/journal.pone.0026777
Stato di pubblicazione	Pubblicato - 2011

Keywords

Lactating Dairy Cows
aflatoxin B1
vaccination

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10.1371/journal.pone.0026777

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title = "Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B-1 Carry Over in Milk",

abstract = "The potential of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB1-KLH) in controlling the carry over of the aflatoxin B1 (AFB1) metabolite aflatoxin M1 (AFM1) in cow milk is reported. AFB1 is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB1 is AFB1 chemically modified as AFB1-1(Ocarboxymethyl) oxime. In comparison to AFB1, AnAFB1 has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB1-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB1 IgG antibodies (Abs) which were cross reactive with AFB1, AFG1, and AFG2. The elicited anti- AFB1 Abs were able to hinder the secretion of AFM1 into the milk of cows continuously fed with AFB1. Vaccination of lactating animals with conjugated AnAFB1 may represent a solution to the public hazard constituted by milk and cheese contaminated",

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AU - Polonelli, Luciano

AU - Giovati, Laura

AU - Magliani, Walter

AU - Conti, Stefania

AU - Sforza, Stefano

AU - Calabretta, Alessandro

AU - Casoli, Claudio

AU - Ronzi, Paola

AU - Grilli, Ester

AU - Gallo, Antonio

AU - Masoero, Francesco

AU - Piva, Gianfranco

PY - 2011

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N2 - The potential of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB1-KLH) in controlling the carry over of the aflatoxin B1 (AFB1) metabolite aflatoxin M1 (AFM1) in cow milk is reported. AFB1 is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB1 is AFB1 chemically modified as AFB1-1(Ocarboxymethyl) oxime. In comparison to AFB1, AnAFB1 has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB1-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB1 IgG antibodies (Abs) which were cross reactive with AFB1, AFG1, and AFG2. The elicited anti- AFB1 Abs were able to hinder the secretion of AFM1 into the milk of cows continuously fed with AFB1. Vaccination of lactating animals with conjugated AnAFB1 may represent a solution to the public hazard constituted by milk and cheese contaminated

AB - The potential of anaflatoxin B1 (AnAFB1) conjugated to keyhole limpet hemocyanin (KLH) as a vaccine (AnAFB1-KLH) in controlling the carry over of the aflatoxin B1 (AFB1) metabolite aflatoxin M1 (AFM1) in cow milk is reported. AFB1 is the most carcinogenic compound in food and foodstuffs amongst aflatoxins (AFs). AnAFB1 is AFB1 chemically modified as AFB1-1(Ocarboxymethyl) oxime. In comparison to AFB1, AnAFB1 has proven to be non-toxic in vitro to human hepatocarcinoma cells and non mutagenic to Salmonella typhimurium strains. AnAFB1-KLH was used for immunization of cows proving to induce a long lasting titer of anti-AFB1 IgG antibodies (Abs) which were cross reactive with AFB1, AFG1, and AFG2. The elicited anti- AFB1 Abs were able to hinder the secretion of AFM1 into the milk of cows continuously fed with AFB1. Vaccination of lactating animals with conjugated AnAFB1 may represent a solution to the public hazard constituted by milk and cheese contaminated

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KW - aflatoxin B1

KW - vaccination

KW - Lactating Dairy Cows

KW - aflatoxin B1

KW - vaccination

UR - http://hdl.handle.net/10807/11630

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Vaccination of Lactating Dairy Cows for the Prevention of Aflatoxin B-1 Carry Over in Milk

Abstract

Keywords

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