TY - JOUR
T1 - V1a AVP receptor is required for neurohypophyseal hormonedependent
differentiation in C2C12 cells
AU - Toschi, Angelica
AU - Murfuni, Ivana
AU - Coletti, Dario
AU - Gambara, Guido
AU - Severi, Anna Lisa
AU - Ramina, Carla
AU - Adamo, Sergio
AU - Scicchitano, Bianca Maria
PY - 2009
Y1 - 2009
N2 - Vasopressin (AVP), oxytocin (OT) and related peptides induce differentiation and hypertrophy
in myogenic cells expressing the V1a-vasopressin receptor (V1aR) or the oxytocin receptor
(OTR). Either receptor can transduce both ligand signals. Binding of AVP and OT to the V1aR
the target cells activates phosphatidylinositol hydrolysis, which in turn releases Ca2+ from
internal stores. The AVP-dependent increase in cytosolic Ca2+ induces the activation of
calcium/calmodulin-dependent kinase (CaMK) and calcineurin signaling, two pathways
required for the full expression of the differentiated phenotype. Here we investigate the role of
V1aR in myogenesis and hypertrophy by ectopically restoring V1aR expression and function
using the C2C12 cell line, which is an experimental model of satellite cells that do not respond
to AVP treatment. Our results show that AVP treatment enhances myogenic differentiation in
V1aR-transfected C2C12 cultures alone. Moreover, calcium imaging analyses performed in
individual control and V1aR-transfected C2C12 cells demonstrated that the presence of V1aR
is sufficient to make C2C12 cells responsive to neurohypophyseal hormones stimulation, as
demonstrated by the rapid and sustained release of calcium from internal stores observed in
V1aR-transfected cells. These data demonstrate that, despite the high levels of OTR expressed
by C2C12 cells, both AVP and OT failed to stimulate the differentiation program, thereby
indicating that the presence of V1aR is essential to mediate the effects of neurohypophyseal
hormones on myogenic differentiation in C2C12 cells.
AB - Vasopressin (AVP), oxytocin (OT) and related peptides induce differentiation and hypertrophy
in myogenic cells expressing the V1a-vasopressin receptor (V1aR) or the oxytocin receptor
(OTR). Either receptor can transduce both ligand signals. Binding of AVP and OT to the V1aR
the target cells activates phosphatidylinositol hydrolysis, which in turn releases Ca2+ from
internal stores. The AVP-dependent increase in cytosolic Ca2+ induces the activation of
calcium/calmodulin-dependent kinase (CaMK) and calcineurin signaling, two pathways
required for the full expression of the differentiated phenotype. Here we investigate the role of
V1aR in myogenesis and hypertrophy by ectopically restoring V1aR expression and function
using the C2C12 cell line, which is an experimental model of satellite cells that do not respond
to AVP treatment. Our results show that AVP treatment enhances myogenic differentiation in
V1aR-transfected C2C12 cultures alone. Moreover, calcium imaging analyses performed in
individual control and V1aR-transfected C2C12 cells demonstrated that the presence of V1aR
is sufficient to make C2C12 cells responsive to neurohypophyseal hormones stimulation, as
demonstrated by the rapid and sustained release of calcium from internal stores observed in
V1aR-transfected cells. These data demonstrate that, despite the high levels of OTR expressed
by C2C12 cells, both AVP and OT failed to stimulate the differentiation program, thereby
indicating that the presence of V1aR is essential to mediate the effects of neurohypophyseal
hormones on myogenic differentiation in C2C12 cells.
KW - vasopressin
KW - vasopressin
UR - http://hdl.handle.net/10807/97044
M3 - Article
SN - 1120-9992
VL - 2009
SP - 229
EP - 236
JO - BASIC AND APPLIED MYOLOGY
JF - BASIC AND APPLIED MYOLOGY
ER -