Abstract
Solid-phase microextraction (SPME) is a new extraction technique with many advantages: small
sample volume, simplicity, quickness and solvent-free. It is mainly applied to environmental
analysis, but is also useful for the extraction of drugs from biological samples. In this paper the
use of SPME is proposed for the determination of methadone and its main metabolite EDDP in
hair by GC–MS. The hair samples were washed, cut into 1-mm segments, and incubated with
Pronase EÒ for 12 h. A 100-mm polydimethylsiloxane (PDMS) film fibre was submerged for 30
min in a diluted solution of the hydrolysis liquid (1:4 with borax buffer) containing methadone-d3
and EDDP-d as internal standards. Once the microextraction was concluded the fibre was directly 3
inserted into the CG injection port. Linearity was found for methadone and EDDP in the range
studied, 1.0–50 ng/mg hair, with correlation coefficients higher than 0.99. Interassay relative
standard deviation (R.S.D) was determined to be less than 13.30% for methadone and less than
8.94% for EDDP, at 3.0 and 30.0 ng/mg. Analytical recoveries were close to 100% for both
compounds on spiked samples. The method was applied to the analysis of real hair samples from
eight patients of a methadone maintenance programme. The concentration of methadone in hair
ranged from 2.45 to 78.10 ng/mg, and for EDDP from 0.98 to 7.76 ng/mg of hair. Ó 2000
Elsevier Science Ireland Ltd. All rights reserved.
Lingua originale | English |
---|---|
pagine (da-a) | 225-232 |
Numero di pagine | 8 |
Rivista | Forensic Science International |
Volume | 107 |
DOI | |
Stato di pubblicazione | Pubblicato - 2000 |
Keywords
- GC/MS
- Hair
- Methadone, EDDP
- SPME