Abstract
We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.
| Lingua originale | Inglese |
|---|---|
| pagine (da-a) | 6189-6193 |
| Numero di pagine | 5 |
| Rivista | Journal of Clinical Microbiology |
| Volume | 43 |
| DOI | |
| Stato di pubblicazione | Pubblicato - 2005 |
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Keywords
- Bacterial Typing Techniques
- Humans
- Microchip Analytical Procedures
- Mycobacterium
- Mycobacterium Infections
- Mycobacterium tuberculosis
- Nanotechnology
- Oligonucleotide Array Sequence Analysis
- Oligonucleotide Probes
- Polymerase Chain Reaction
- RNA, Ribosomal, 16S
- Time Factors
- Tuberculosis
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