Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria

Maurizio Sanguinetti; Linda Novarese; Brunella Posteraro; Stefania Ranno; Elena De Carolis; Giovanni Pecorini; Barbara Lucignano; Fausta Ardito; Giovanni Fadda

doi:10.1128/JCM.43.12.6189-6193.2005

Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria

Maurizio Sanguinetti, Linda Novarese, Brunella Posteraro, Stefania Ranno, Elena De Carolis, Giovanni Pecorini, Barbara Lucignano, Fausta Ardito, Giovanni Fadda

Risultato della ricerca: Contributo in rivista › Articolo › peer review

7 Citazioni (Scopus)

Abstract

We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.

Lingua originale	Inglese
pagine (da-a)	6189-6193
Numero di pagine	5
Rivista	Journal of Clinical Microbiology
Volume	43
DOI	https://doi.org/10.1128/JCM.43.12.6189-6193.2005
Stato di pubblicazione	Pubblicato - 2005

Keywords

Bacterial Typing Techniques
Humans
Microchip Analytical Procedures
Mycobacterium
Mycobacterium Infections
Mycobacterium tuberculosis
Nanotechnology
Oligonucleotide Array Sequence Analysis
Oligonucleotide Probes
Polymerase Chain Reaction
RNA, Ribosomal, 16S
Time Factors
Tuberculosis

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10.1128/JCM.43.12.6189-6193.2005

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title = "Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria",

abstract = "We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.",

keywords = "Bacterial Typing Techniques, Humans, Microchip Analytical Procedures, Mycobacterium, Mycobacterium Infections, Mycobacterium tuberculosis, Nanotechnology, Oligonucleotide Array Sequence Analysis, Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Time Factors, Tuberculosis, Bacterial Typing Techniques, Humans, Microchip Analytical Procedures, Mycobacterium, Mycobacterium Infections, Mycobacterium tuberculosis, Nanotechnology, Oligonucleotide Array Sequence Analysis, Oligonucleotide Probes, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Time Factors, Tuberculosis",

author = "Maurizio Sanguinetti and Linda Novarese and Brunella Posteraro and Stefania Ranno and \{De Carolis\}, Elena and Giovanni Pecorini and Barbara Lucignano and Fausta Ardito and Giovanni Fadda",

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doi = "10.1128/JCM.43.12.6189-6193.2005",

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T1 - Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria

AU - Sanguinetti, Maurizio

AU - Novarese, Linda

AU - Posteraro, Brunella

AU - Ranno, Stefania

AU - De Carolis, Elena

AU - Pecorini, Giovanni

AU - Lucignano, Barbara

AU - Ardito, Fausta

AU - Fadda, Giovanni

PY - 2005

Y1 - 2005

N2 - We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.

AB - We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.

KW - Bacterial Typing Techniques

KW - Humans

KW - Microchip Analytical Procedures

KW - Mycobacterium

KW - Mycobacterium Infections

KW - Mycobacterium tuberculosis

KW - Nanotechnology

KW - Oligonucleotide Array Sequence Analysis

KW - Oligonucleotide Probes

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal, 16S

KW - Time Factors

KW - Tuberculosis

KW - Bacterial Typing Techniques

KW - Humans

KW - Microchip Analytical Procedures

KW - Mycobacterium

KW - Mycobacterium Infections

KW - Mycobacterium tuberculosis

KW - Nanotechnology

KW - Oligonucleotide Array Sequence Analysis

KW - Oligonucleotide Probes

KW - Polymerase Chain Reaction

KW - RNA, Ribosomal, 16S

KW - Time Factors

KW - Tuberculosis

UR - http://hdl.handle.net/10807/17410

U2 - 10.1128/JCM.43.12.6189-6193.2005

DO - 10.1128/JCM.43.12.6189-6193.2005

M3 - Article

SN - 0095-1137

VL - 43

SP - 6189

EP - 6193

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

ER -

Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria

Abstract

Keywords

OSS delle Nazioni Unite

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