Use of microelectronic array technology for rapid identification of clinically relevant mycobacteria

Maurizio Sanguinetti, Brunella Posteraro, Giovanni Pecorini, Giovanni Fadda, Linda Novarese, Stefania Ranno, Elena De Carolis, Barbara Lucignano, Fausta Ardito

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

7 Citazioni (Scopus)

Abstract

We developed a new method based on the Nanochip microelectronic array technology for identification of various clinically relevant mycobacterial species. PCR-amplified rRNA genes obtained from 270 positive Mycobacteria Growth Indicator Tube cultures were successfully tested by hybridizing them with species-selective probes, and the results agreed with those of conventional identification methods. The system is rapid and accurate and opens new perspectives in clinical diagnostics.
Lingua originaleEnglish
pagine (da-a)6189-6193
Numero di pagine5
RivistaJournal of Clinical Microbiology
Volume43
DOI
Stato di pubblicazionePubblicato - 2005

Keywords

  • Bacterial Typing Techniques
  • Humans
  • Microchip Analytical Procedures
  • Mycobacterium
  • Mycobacterium Infections
  • Mycobacterium tuberculosis
  • Nanotechnology
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S
  • Time Factors
  • Tuberculosis

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