Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species

Elena De Carolis; Antonietta Vella; Ada R. Florio; Patrizia Posteraro; David S. Perlin; Maurizio Sanguinetti; Brunella Posteraro

doi:10.1128/JCM.00224-12

Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species

Elena De Carolis, Antonietta Vella, Ada R. Florio, Patrizia Posteraro, David S. Perlin, Maurizio Sanguinetti, Brunella Posteraro

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

84 Citazioni (Scopus)

Abstract

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.

Lingua originale	English
pagine (da-a)	2479-2483
Numero di pagine	5
Rivista	Journal of Clinical Microbiology
Volume	50
DOI	https://doi.org/10.1128/JCM.00224-12
Stato di pubblicazione	Pubblicato - 2012

Keywords

Caspofungin

Accesso al documento

10.1128/JCM.00224-12

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title = "Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species",

abstract = "Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.",

keywords = "Caspofungin, Caspofungin",

author = "{De Carolis}, Elena and Antonietta Vella and Florio, {Ada R.} and Patrizia Posteraro and Perlin, {David S.} and Maurizio Sanguinetti and Brunella Posteraro",

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doi = "10.1128/JCM.00224-12",

language = "English",

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Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species. / De Carolis, Elena; Vella, Antonietta; Florio, Ada R. et al.
In: Journal of Clinical Microbiology, Vol. 50, 2012, pag. 2479-2483.

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

TY - JOUR

T1 - Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species

AU - De Carolis, Elena

AU - Vella, Antonietta

AU - Florio, Ada R.

AU - Posteraro, Patrizia

AU - Perlin, David S.

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

PY - 2012

Y1 - 2012

N2 - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.

AB - Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for testing susceptibility to caspofungin of wild-type and fks mutant isolates of Candida and Aspergillus. Complete essential agreement was observed with the CLSI reference method, with categorical agreement for 94.1% of the Candida isolates tested. Thus, MALDI-TOF MS is a reliable and accurate method to detect fungal isolates with reduced caspofungin susceptibility.

KW - Caspofungin

UR - http://hdl.handle.net/10807/13008

U2 - 10.1128/JCM.00224-12

DO - 10.1128/JCM.00224-12

M3 - Article

SN - 1098-660X

VL - 50

SP - 2479

EP - 2483

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

ER -

Use of matrix-assisted laser desorption ionization-time of flight mass spectrometry for caspofungin susceptibility testing of Candida and Aspergillus species

Abstract

Keywords

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