Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening

Adriano Marocco, Jamila Bernardi, Giorgia Carletti, Concetta Licciardello, M. Patrizia Russo, M. Luisa Chiusano, Giuseppe Reforgiato Recupero

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

19 Citazioni (Scopus)

Abstract

A flesh specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. Thearray included 301 probes derived from a subtracted SSH library,a cDNA–AFLP collection,and a set of regulatory genes from the Harvest citrus database. The custom array was hybridized using samples of Moro,a pigmented cultivar,and Cadenera,a common cultivar,at three different ripening stages:the immature phase,the half way point of maturation(corresponding to the start of Moro pigmentation)and the full ripening. Of the 301probes,27 in total,corresponding to 20 different transcripts,indicated differential expression in stage-to-stage and/or cultivar-to-cultivar comparisons.Transcripts encoding for anthocyanin biosynthesis represented most of the total over- expressed probes.The remaining differentially expressed transcripts were functionally associated with primary metabolism as flavor biosynthesis,defense and signal transduction.The expressed products associated with probes indicating differential expression were confirmed by qRT-PCR. The microarray was designed considering a small collection of sequences useful for monitoring specific pathways and regulatory genes related to fruit ripening and anthocyanin pigmentation.The main novelty of this customization is the use of expressed sequences specifically derived from blood orange flesh to study different cultivars and ripening stages,and the provision of further information about processes related to anthocyanin pigmentation in citrus fruit flesh.
Lingua originaleEnglish
pagine (da-a)301-310
Numero di pagine10
RivistaJournal of Plant Physiology
Volume167
Stato di pubblicazionePubblicato - 2010

Keywords

  • microarray
  • orange
  • transcriptome analysis

Fingerprint Entra nei temi di ricerca di 'Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening'. Insieme formano una fingerprint unica.

Cita questo