TY - JOUR
T1 - Unraveling the different proteomic platforms
AU - Messana, I
AU - Cabras, T
AU - Iavarone, Federica
AU - Vincenzoni, Federica
AU - Urbani, Andrea
AU - Castagnola, Massimo
PY - 2013
Y1 - 2013
N2 - This review is addressed to scientists working outside the field of proteomics and wishes to shed a light on the possibility offered by the latest proteomics strategies. Bottom-up and top-down platforms are critically examined outlining advantages and limitations of their application to qualitative and quantitative investigations. Discovery, directed and targeted proteomics as different options for the management of the MS instrument are defined emphasizing their integration in the experimental plan to accomplish meaningful results. The issue of data validation is analyzed and discussed. The most common qualitative proteomic platforms are described, with a particular emphasis on enrichment methods to elucidate PTMs codes (i.e. ubiquitin and histone codes). Label-free and labeled methods for relative and absolute quantification are critically compared. The possible contribution of proteomics platforms to the transition from structural proteomics to functional proteomics (study of the functional connections between different proteins) and to the challenging system biology (integrated study of all the functional cellular functions) is also briefly discussed.
AB - This review is addressed to scientists working outside the field of proteomics and wishes to shed a light on the possibility offered by the latest proteomics strategies. Bottom-up and top-down platforms are critically examined outlining advantages and limitations of their application to qualitative and quantitative investigations. Discovery, directed and targeted proteomics as different options for the management of the MS instrument are defined emphasizing their integration in the experimental plan to accomplish meaningful results. The issue of data validation is analyzed and discussed. The most common qualitative proteomic platforms are described, with a particular emphasis on enrichment methods to elucidate PTMs codes (i.e. ubiquitin and histone codes). Label-free and labeled methods for relative and absolute quantification are critically compared. The possible contribution of proteomics platforms to the transition from structural proteomics to functional proteomics (study of the functional connections between different proteins) and to the challenging system biology (integrated study of all the functional cellular functions) is also briefly discussed.
KW - proteomic
KW - proteomic platforms
KW - proteomic
KW - proteomic platforms
UR - https://publicatt.unicatt.it/handle/10807/40718
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84872116116&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84872116116&origin=inward
U2 - 10.1002/jssc.201200830
DO - 10.1002/jssc.201200830
M3 - Article
SN - 1615-9314
VL - 36
SP - 128
EP - 139
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 1
ER -