Unraveling the different proteomic platforms

Federica Iavarone, Andrea Urbani, Federica Vincenzoni, Massimo Castagnola, Irene Messana, Tiziana Cabras

Risultato della ricerca: Contributo in rivistaArticolo in rivista

40 Citazioni (Scopus)

Abstract

This review is addressed to scientists working outside the field of proteomics and wishes to shed a light on the possibility offered by the latest proteomics strategies. Bottom-up and top-down platforms are critically examined outlining advantages and limitations of their application to qualitative and quantitative investigations. Discovery, directed and targeted proteomics as different options for the management of the MS instrument are defined emphasizing their integration in the experimental plan to accomplish meaningful results. The issue of data validation is analyzed and discussed. The most common qualitative proteomic platforms are described, with a particular emphasis on enrichment methods to elucidate PTMs codes (i.e. ubiquitin and histone codes). Label-free and labeled methods for relative and absolute quantification are critically compared. The possible contribution of proteomics platforms to the transition from structural proteomics to functional proteomics (study of the functional connections between different proteins) and to the challenging system biology (integrated study of all the functional cellular functions) is also briefly discussed.
Lingua originaleEnglish
pagine (da-a)128-139
Numero di pagine12
RivistaJournal of Separation Science
Volume36
DOI
Stato di pubblicazionePubblicato - 2013

Keywords

  • proteomic
  • proteomic platforms

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