TY - JOUR
T1 - Ultra high performance liquid chromatography–electrospray ionization–tandem mass spectrometry screening method for direct analysis of designer drugs, “spice” and stimulants in oral fluid
AU - Strano Rossi, Sabina
AU - Anzillotti, Luca
AU - Castrignanò, Erika
AU - Romolo, Francesco Saverio
AU - Chiarotti, Marcello
PY - 2012
Y1 - 2012
N2 - An ultra high performance liquid chromatography–electrospray ionization–tandem mass spectrometry
(UHPLC–ESI–MS/MS) screening method for the direct analysis in oral fluid (OF) of 24 drugs, including
new synthetic cannabinoids and so-called “smart” designer drugs, in a single chromatographic run
was set up. Benzylpiperazine, methylone, 5,6-methylenedioxy-2-aminoindane (MDAI), fenproporex,
4-fluoroamphetamine (4-FA), 4-methyl-N-ethylcathinone (4-MEC), 4-methylamphetamine (4-MA),
methylbenzodioxolylbutanamine (MBDB), mephedrone, methylthioamphetamine (MTA), methylenedioxypyrovalerone
(MDPV), mefenorex, nabilone, furfenorex, clobenzorex, JWH-200, AM 694, JWH-250,
JWH-073, JWH-018, JWH-019, JWH-122, HU 210 and CP 47497 were determined in a chromatographic
run of 9 min only with no sample pre-treatment, after addition of ISs and dilution in mobile phase A.
This method is designed to be applied to 250 L of OF sample, anyway is suitable to be used on smaller
volumes (till 100 L). LODs vary from 1 ng/mL to 20 ng/mL. No interfering peaks were observed due to
similar analytes, common therapeutic drugs or endogenous compounds. Matrix effect, although present
especially for mephedrone, is acceptable, allowing the detection of the compounds at the LODs described.
The developed method was applied on 400 real OF samples from on-site tests performed by police officers.
AB - An ultra high performance liquid chromatography–electrospray ionization–tandem mass spectrometry
(UHPLC–ESI–MS/MS) screening method for the direct analysis in oral fluid (OF) of 24 drugs, including
new synthetic cannabinoids and so-called “smart” designer drugs, in a single chromatographic run
was set up. Benzylpiperazine, methylone, 5,6-methylenedioxy-2-aminoindane (MDAI), fenproporex,
4-fluoroamphetamine (4-FA), 4-methyl-N-ethylcathinone (4-MEC), 4-methylamphetamine (4-MA),
methylbenzodioxolylbutanamine (MBDB), mephedrone, methylthioamphetamine (MTA), methylenedioxypyrovalerone
(MDPV), mefenorex, nabilone, furfenorex, clobenzorex, JWH-200, AM 694, JWH-250,
JWH-073, JWH-018, JWH-019, JWH-122, HU 210 and CP 47497 were determined in a chromatographic
run of 9 min only with no sample pre-treatment, after addition of ISs and dilution in mobile phase A.
This method is designed to be applied to 250 L of OF sample, anyway is suitable to be used on smaller
volumes (till 100 L). LODs vary from 1 ng/mL to 20 ng/mL. No interfering peaks were observed due to
similar analytes, common therapeutic drugs or endogenous compounds. Matrix effect, although present
especially for mephedrone, is acceptable, allowing the detection of the compounds at the LODs described.
The developed method was applied on 400 real OF samples from on-site tests performed by police officers.
KW - DUID,
KW - Forensic toxicology,
KW - Oral fluid
KW - UHPLC–MS/MS
KW - smart drugs,
KW - synthetic cannabinoids
KW - DUID,
KW - Forensic toxicology,
KW - Oral fluid
KW - UHPLC–MS/MS
KW - smart drugs,
KW - synthetic cannabinoids
UR - http://hdl.handle.net/10807/28314
UR - http://dx.doi.org/10.1016/j.chroma.2012.07.098,
U2 - 10.1016/j.chroma.2012.07.098
DO - 10.1016/j.chroma.2012.07.098
M3 - Article
SN - 0021-9673
VL - 2012
SP - 37
EP - 42
JO - Journal of Chromatography A
JF - Journal of Chromatography A
ER -