TY - JOUR
T1 - UHPLC-ESI-MS/MS method for direct analysis of drugs of abuse in oral fluid for DUID assessment
AU - Strano Rossi, Sabina
AU - Anzillotti, Luca
AU - Castrignanò, Erika
AU - Felli, Maria Linda
AU - Serpelloni, Giovanni
AU - Mollica, Roberto
AU - Chiarotti, Marcello
PY - 2011
Y1 - 2011
N2 - An ultra-high-performance liquid chromatography–
electrospray ionization–tandem mass spectrometry method for
the direct analysis in oral fluid (OF) of several abused drugs and
metabolites in a single chromatographic run was set up and
validated. Amphetamine, methamphetamine, morphine, O-6-
monoacetylmorphine, cocaine, codeine, methylenedioxymethamphetamine
(MDMA), methylenedioxyethylamphetamine,
methylenedioxyamphetamine, methadone,
benzoylecgonine (BEG), Ä9-tetrahydrocannabinol (THC),
ketamine, and cocaethylene were determined in a single
chromatographic run with no sample pretreatment, after
addition of the respective deuterated internal standards. The
method was designed to perform a confirmation analysis on
the residual OF samples after the preliminary on-site screening
test, and it was applied on preservative buffers from different
devices (Mavand Rapidstat, Concateno DDS, and Greiner
Bio-One) or on neat OF samples. The method was suitable to
be applied to the small amounts of sample available for the
confirmatory analysis after the preliminary on-site screening
or on undiluted OF samples. Limits of detection varied from 5
(morphine) to 0.2 ng/mL (methamphetamine, MDMA, BEG,
and cocaethylene). The method was linear for all the
substances involved, giving quadratic correlation coefficients
of >0.99 in all the different preservative buffers checked. In addition, repeatability and accuracy were satisfactory for the
majority of the substances, except for a few cases. The
developed method was subsequently applied to 466 residual
samples from on-site screening performed by police officers.
Of these samples, 74 showed the presence of cocaine and
metabolites; THC was detected in 49 samples. Two samples
showed codeine and morphine while MDMA was detected in
11 samples and ketamine in four samples.
AB - An ultra-high-performance liquid chromatography–
electrospray ionization–tandem mass spectrometry method for
the direct analysis in oral fluid (OF) of several abused drugs and
metabolites in a single chromatographic run was set up and
validated. Amphetamine, methamphetamine, morphine, O-6-
monoacetylmorphine, cocaine, codeine, methylenedioxymethamphetamine
(MDMA), methylenedioxyethylamphetamine,
methylenedioxyamphetamine, methadone,
benzoylecgonine (BEG), Ä9-tetrahydrocannabinol (THC),
ketamine, and cocaethylene were determined in a single
chromatographic run with no sample pretreatment, after
addition of the respective deuterated internal standards. The
method was designed to perform a confirmation analysis on
the residual OF samples after the preliminary on-site screening
test, and it was applied on preservative buffers from different
devices (Mavand Rapidstat, Concateno DDS, and Greiner
Bio-One) or on neat OF samples. The method was suitable to
be applied to the small amounts of sample available for the
confirmatory analysis after the preliminary on-site screening
or on undiluted OF samples. Limits of detection varied from 5
(morphine) to 0.2 ng/mL (methamphetamine, MDMA, BEG,
and cocaethylene). The method was linear for all the
substances involved, giving quadratic correlation coefficients
of >0.99 in all the different preservative buffers checked. In addition, repeatability and accuracy were satisfactory for the
majority of the substances, except for a few cases. The
developed method was subsequently applied to 466 residual
samples from on-site screening performed by police officers.
Of these samples, 74 showed the presence of cocaine and
metabolites; THC was detected in 49 samples. Two samples
showed codeine and morphine while MDMA was detected in
11 samples and ketamine in four samples.
KW - Forensic toxicology . Oral fluid UHPLC-MS/MS DUID
KW - Forensic toxicology . Oral fluid UHPLC-MS/MS DUID
UR - http://hdl.handle.net/10807/2791
U2 - 10.1007/s00216-011-5108-y
DO - 10.1007/s00216-011-5108-y
M3 - Article
SN - 1618-2642
VL - 2011
SP - 609
EP - 624
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
ER -