UDP exerts cytostatic and cytotoxic actions in human neuroblastoma SH-SY5Y cells over-expressing P2Y6 receptor

S Apolloni, P Finocchi, I D'Agnano, S Alloisio, M Nobile, Nadia D'Ambrosi, C. Volonté

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

9 Citazioni (Scopus)


The role of P2 receptors for purines/pyrimidines is not well characterized in neuroblastoma, although a variety of purinergic mRNAs/proteins are expressed in these cells. Among these, the P2Y(6) receptor is the only subtype distinguished by UDP-specific activation. In this work, after over-expressing the P2Y(6) protein in human neuroblastoma SH-SY5Y cells, we find that UDP arrests cell cycle and induces apoptosis, by counteracting the pathological functioning of neuroblastoma in vitro. UDP also causes mitochondrial damage through diffusion of cytochrome c in the cytoplasm, and stimulates caspase-3,7,8 activities, with extensive over-expression of manganese superoxide dismutase. Our data establish the direct toxic role and anti-cancer activity of UDP in a neuroblastoma cell line, and identify the P2Y(6) receptor as a novel potential target in anti-tumoural therapies. This constitutes an advancement not only in the knowledge of purinergic signalling, but also in the biological and pathological aspects of neuroblastoma in vitro.
Lingua originaleEnglish
pagine (da-a)670-678
Numero di pagine9
RivistaNeurochemistry International
Stato di pubblicazionePubblicato - 2010
Pubblicato esternamente


  • Annexin A5
  • Antineoplastic Agents
  • Apoptosis
  • Blotting, Western
  • Calcium
  • Caspase 3
  • Caspase 7
  • Cell Count
  • Cell Line, Tumor
  • Cell Survival
  • Cloning, Molecular
  • Cytostatic Agents
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Microscopy, Fluorescence
  • Plasmids
  • RNA, Neoplasm
  • Receptors, Purinergic P2
  • Reverse Transcriptase Polymerase Chain Reaction
  • Uridine Diphosphate


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