TY - JOUR
T1 - Trigeminal satellite cells modulate neuronal responses to triptans: relevance for migraine therapy.
AU - De Corato, Alice
AU - Capuano, Alessandro
AU - Curro', Diego
AU - Tringali, Giuseppe
AU - Navarra, Pierluigi
AU - Dello Russo, Cinzia
PY - 2011
Y1 - 2011
N2 - In the present paper, we have further developed an in vitro model to study neuronal-glial interaction at trigeminal level by characterizing the effects of conditioned medium (CM) collected from activated primary cultures of satellite glial cells (SGCs) on calcitonin gene-related peptide (CGRP) release from rat trigeminal neurons. Moreover, we investigated whether such release is inhibited by a clinically relevant anti-migraine drug, sumatriptan. CM effects were tested on trigeminal neuronal cultures in different conditions of activation and at different time points. Long-term exposures of trigeminal neurons to CM increased directly neuronal CGRP release, which was further enhanced by the exposure to capsaicin. In this framework, the anti-migraine drug sumatriptan was able to inhibit the evoked CGRP release from naïve trigeminal neuron cultures, as well as from trigeminal cultures pre-exposed for 30 min to CM. On the contrary, sumatriptan failed to inhibit evoked CGRP release from trigeminal neurons after prolonged (4 and 8 h) pre-exposures to CM. These findings were confirmed in co-culture experiments (neurons and SGCs), where activation of SGCs or a bradykinin priming were used. Our data demonstrate that SGCs activation could influence neuronal excitability, and that this event affects the neuronal responses to triptans.
AB - In the present paper, we have further developed an in vitro model to study neuronal-glial interaction at trigeminal level by characterizing the effects of conditioned medium (CM) collected from activated primary cultures of satellite glial cells (SGCs) on calcitonin gene-related peptide (CGRP) release from rat trigeminal neurons. Moreover, we investigated whether such release is inhibited by a clinically relevant anti-migraine drug, sumatriptan. CM effects were tested on trigeminal neuronal cultures in different conditions of activation and at different time points. Long-term exposures of trigeminal neurons to CM increased directly neuronal CGRP release, which was further enhanced by the exposure to capsaicin. In this framework, the anti-migraine drug sumatriptan was able to inhibit the evoked CGRP release from naïve trigeminal neuron cultures, as well as from trigeminal cultures pre-exposed for 30 min to CM. On the contrary, sumatriptan failed to inhibit evoked CGRP release from trigeminal neurons after prolonged (4 and 8 h) pre-exposures to CM. These findings were confirmed in co-culture experiments (neurons and SGCs), where activation of SGCs or a bradykinin priming were used. Our data demonstrate that SGCs activation could influence neuronal excitability, and that this event affects the neuronal responses to triptans.
KW - CGRP
KW - migraine
KW - neuronal sensitization
KW - sumatriptan
KW - trigeminal satellite cells
KW - CGRP
KW - migraine
KW - neuronal sensitization
KW - sumatriptan
KW - trigeminal satellite cells
UR - https://publicatt.unicatt.it/handle/10807/6858
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84871279046&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84871279046&origin=inward
U2 - 10.1017/S1740925X11000172
DO - 10.1017/S1740925X11000172
M3 - Article
SN - 1740-925X
VL - 7
SP - 109
EP - 116
JO - Neuron Glia Biology
JF - Neuron Glia Biology
IS - 2-4
ER -