Transferof plasmid-mediated resistance totetracycline in pathogenic bacteria from¢sh and aquaculture environments

Lorenzo Morelli, Elena Guglielmetti, J. M. Korhonen, J. Heikkinen, A. Von Wright

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

The transferability of a large plasmid that harbors a tetracycline resistance gene tet(S), to fish and human pathogens was assessed using electrotransformation and conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients (Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore the tetracycline resistance determinant was transferred via electroporation to L. garvieae. A transformant clone was used as the donor in conjugation experiments with three different L. monocytogenes strains. To our knowledge, this is the first study showing the transfer of an antibiotic resistance plasmid from fishassociated lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not the original host of the tetracycline resistance but experimentally created by electroporation. These results demonstrate that the antibiotic resistance genes in the fish intestinal bacteria have the potential to spread both to
Lingua originaleEnglish
pagine (da-a)28-34
Numero di pagine7
RivistaFEMS MICROBIOLOGY LETTERS
Stato di pubblicazionePubblicato - 2009

Keywords

  • Lactococcus garvieae
  • Lactococcus spp
  • Listeria monocytogenes
  • aquaculture
  • horizontal gene transfer
  • tetracycline resistance

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