TY - JOUR
T1 - Transferof plasmid-mediated resistance totetracycline in pathogenic bacteria from¢sh and aquaculture environments
AU - Morelli, Lorenzo
AU - Guglielmetti, Elena
AU - 32937,
AU - FACOLTA' DI SCIENZE AGRARIE, ALIMENTARI E AMBIENTALI
AU - (DiSTAS), PIACENZA - Dipartimento di Scienze e tecnologie alimentari per una filiera agro-alimentare sostenibile
AU - Korhonen, J. M.
AU - Heikkinen, J.
AU - Von Wright, A.
PY - 2009
Y1 - 2009
N2 - The transferability of a large plasmid that harbors a tetracycline resistance gene
tet(S), to fish and human pathogens was assessed using electrotransformation and
conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis
ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients
(Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore
the tetracycline resistance determinant was transferred via electroporation to
L. garvieae. A transformant clone was used as the donor in conjugation
experiments with three different L. monocytogenes strains. To our knowledge, this
is the first study showing the transfer of an antibiotic resistance plasmid from fishassociated
lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not
the original host of the tetracycline resistance but experimentally created by
electroporation. These results demonstrate that the antibiotic resistance genes in
the fish intestinal bacteria have the potential to spread both to
AB - The transferability of a large plasmid that harbors a tetracycline resistance gene
tet(S), to fish and human pathogens was assessed using electrotransformation and
conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis
ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients
(Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore
the tetracycline resistance determinant was transferred via electroporation to
L. garvieae. A transformant clone was used as the donor in conjugation
experiments with three different L. monocytogenes strains. To our knowledge, this
is the first study showing the transfer of an antibiotic resistance plasmid from fishassociated
lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not
the original host of the tetracycline resistance but experimentally created by
electroporation. These results demonstrate that the antibiotic resistance genes in
the fish intestinal bacteria have the potential to spread both to
KW - Lactococcus garvieae
KW - Lactococcus spp
KW - Listeria monocytogenes
KW - aquaculture
KW - horizontal gene transfer
KW - tetracycline resistance
KW - Lactococcus garvieae
KW - Lactococcus spp
KW - Listeria monocytogenes
KW - aquaculture
KW - horizontal gene transfer
KW - tetracycline resistance
UR - http://hdl.handle.net/10807/28101
M3 - Article
SP - 28
EP - 34
JO - FEMS MICROBIOLOGY LETTERS
JF - FEMS MICROBIOLOGY LETTERS
SN - 0378-1097
ER -