Abstract
The transferability of a large plasmid that harbors a tetracycline resistance gene
tet(S), to fish and human pathogens was assessed using electrotransformation and
conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis
ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients
(Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore
the tetracycline resistance determinant was transferred via electroporation to
L. garvieae. A transformant clone was used as the donor in conjugation
experiments with three different L. monocytogenes strains. To our knowledge, this
is the first study showing the transfer of an antibiotic resistance plasmid from fishassociated
lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not
the original host of the tetracycline resistance but experimentally created by
electroporation. These results demonstrate that the antibiotic resistance genes in
the fish intestinal bacteria have the potential to spread both to
Lingua originale | English |
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pagine (da-a) | 28-34 |
Numero di pagine | 7 |
Rivista | FEMS Microbiology Letters |
Stato di pubblicazione | Pubblicato - 2009 |
Keywords
- Lactococcus garvieae
- Lactococcus spp
- Listeria monocytogenes
- aquaculture
- horizontal gene transfer
- tetracycline resistance