Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

Claudia Martelli; Luca D'Angelo; Marta Barba; Mirko Baranzini; Ilaria Inserra; Federica Iavarone; Federica Vincenzoni; Gianpiero Tamburrini; Luca Massimi; Concezio Di Rocco; Massimo Caldarelli; Irene Messana; Fabrizio Michetti; Massimo Castagnola; Wanda Lattanzi; Claudia Desiderio

doi:10.1016/j.euprot.2016.03.015

Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

Claudia Martelli, Luca D'Angelo, Marta Barba, Mirko Baranzini, Ilaria Inserra, Federica Iavarone, Federica Vincenzoni, Gianpiero Tamburrini, Luca Massimi, Concezio Di Rocco, Massimo Caldarelli, Irene Messana, Fabrizio Michetti, Massimo Castagnola, Wanda Lattanzi, Claudia Desiderio

Risultato della ricerca: Contributo in rivista › Articolo in rivista

3 Citazioni (Scopus)

Abstract

The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome.

Lingua originale	English
pagine (da-a)	13-21
Numero di pagine	9
Rivista	EuPA Open Proteomics
Volume	12
DOI	https://doi.org/10.1016/j.euprot.2016.03.015
Stato di pubblicazione	Pubblicato - 2016

Keywords

Biochemistry
DAOY cell line
Medulloblastoma
Peptidome
Proteome
Top-down proteomics

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10.1016/j.euprot.2016.03.015

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Martelli, C., D'Angelo, L., Barba, M., Baranzini, M., Inserra, I., Iavarone, F., Vincenzoni, F., Tamburrini, G., Massimi, L., Di Rocco, C., Caldarelli, M., Messana, I., Michetti, F., Castagnola, M., Lattanzi, W., & Desiderio, C. (2016). Top-down proteomic characterization of DAOY medulloblastoma tumor cell line. EuPA Open Proteomics, 12, 13-21. https://doi.org/10.1016/j.euprot.2016.03.015

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title = "Top-down proteomic characterization of DAOY medulloblastoma tumor cell line",

abstract = "The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome.",

keywords = "Biochemistry, DAOY cell line, Medulloblastoma, Peptidome, Proteome, Top-down proteomics, Biochemistry, DAOY cell line, Medulloblastoma, Peptidome, Proteome, Top-down proteomics",

author = "Claudia Martelli and Luca D'Angelo and Marta Barba and Mirko Baranzini and Ilaria Inserra and Federica Iavarone and Federica Vincenzoni and Gianpiero Tamburrini and Luca Massimi and {Di Rocco}, Concezio and Massimo Caldarelli and Irene Messana and Fabrizio Michetti and Massimo Castagnola and Wanda Lattanzi and Claudia Desiderio",

year = "2016",

doi = "10.1016/j.euprot.2016.03.015",

language = "English",

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Martelli, C, D'Angelo, L, Barba, M, Baranzini, M, Inserra, I, Iavarone, F, Vincenzoni, F, Tamburrini, G , Massimi, L, Di Rocco, C, Caldarelli, M, Messana, I, Michetti, F, Castagnola, M, Lattanzi, W & Desiderio, C 2016, 'Top-down proteomic characterization of DAOY medulloblastoma tumor cell line', EuPA Open Proteomics, vol. 12, pagg. 13-21. https://doi.org/10.1016/j.euprot.2016.03.015

TY - JOUR

T1 - Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

AU - Martelli, Claudia

AU - D'Angelo, Luca

AU - Barba, Marta

AU - Baranzini, Mirko

AU - Inserra, Ilaria

AU - Iavarone, Federica

AU - Vincenzoni, Federica

AU - Tamburrini, Gianpiero

AU - Massimi, Luca

AU - Di Rocco, Concezio

AU - Caldarelli, Massimo

AU - Messana, Irene

AU - Michetti, Fabrizio

AU - Castagnola, Massimo

AU - Lattanzi, Wanda

AU - Desiderio, Claudia

PY - 2016

Y1 - 2016

N2 - The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome.

AB - The proteome of the DAOY medulloblastoma cell line has been investigated by an LC-MS top-down platform. This approach, unlike the bottom-up one, allows identifying proteins and peptides in their intact forms, and disclosing post-translational modifications, proteoforms and naturally occurring peptides. Indeed, 25 out of the 53 proteins identified, were not previously characterized in DAOY cells. Most of them were functionally interconnected, being mainly involved in binding, catalytic and structural activities, and metabolic processes. The top-down approach, applied in this preliminary study, disclosed the presence of several naturally occurring peptide fragments that strongly characterized DAOY cell intact proteome.

KW - Biochemistry

KW - DAOY cell line

KW - Medulloblastoma

KW - Peptidome

KW - Proteome

KW - Top-down proteomics

KW - Biochemistry

KW - DAOY cell line

KW - Medulloblastoma

KW - Peptidome

KW - Proteome

KW - Top-down proteomics

UR - http://hdl.handle.net/10807/124196

UR - http://www.journals.elsevier.com/eupa-open-proteomics/

U2 - 10.1016/j.euprot.2016.03.015

DO - 10.1016/j.euprot.2016.03.015

M3 - Article

SN - 2212-9685

VL - 12

SP - 13

EP - 21

JO - EuPA Open Proteomics

JF - EuPA Open Proteomics

ER -

Top-down proteomic characterization of DAOY medulloblastoma tumor cell line

Abstract

Keywords

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