The use of immobilized pH gradients for the detection of human polymorphisms in the forensic identification of bloodstains

Vincenzo Lorenzo Pascali, I Munoz Barus, A Carracedo

Risultato della ricerca: Contributo in rivistaArticolo in rivista

1 Citazioni (Scopus)

Abstract

Some polymorphic proteins (alpha 1-antitrypsin, orosomucoid, transferrin, group specific component, plasminogen) and enzymes (phosphoglucomutase, acid phosphatase, estrase D) were determined in bloodstain extracts by isoelectric focusing with carrier ampholytes (CA) and with immobilized pH gradients (IPGs) rehydrated with CA. IPGs yield superior results for typing of genetics markers in bloodstains since phenotypes are better distinguished and the bands are straighter and sharper in the presence of contaminants. Also, the sensitivity of IPGs with CA is similar to isoelectric focusing (IEF) with CA. A new variant, ACP*B1, found in Negroid west African populations and not found in Caucasians is described. Such a variant can only be determined by IPGs since its isoelectric point (pI 5.95) is close to that of the ACP*B (pI 6.05) variant.
Lingua originaleEnglish
pagine (da-a)239-243
Numero di pagine5
RivistaElectrophoresis
Volume13
Stato di pubblicazionePubblicato - 1992

Keywords

  • Ampholyte Mixtures/chemistry
  • Blood Proteins/genetics
  • Forensic Medicine
  • Hydrogen-Ion Concentration

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