The secretion and maturation of prosaposin and procathepsin D are blocked in embryonic neural progenitor cells

Ruggero De Maria Marchiano, Rosa Salvioli, Lucia Ricci-Vitiani, Massimo Tatti, Susanna Scarpa, Anna Maria Vaccaro

Risultato della ricerca: Contributo in rivistaArticolo in rivista

9 Citazioni (Scopus)

Abstract

The notion that prosaposin (Prosap) is likely involved in brain development and regeneration led us to explore its expression in stem/progenitor neural cells and its fate after cell differentiation. The expression of procathepsin-cathepsin D (proCath-Cath D), an endoprotease that plays an important role in the processing and sorting of Prosap, has been concomitantly examined. Our data evidenced that in embryonic human neural progenitor cells (eHNPCs) intact and high molecular weight intermediate forms of Prosap and intermediate forms of Cath D accumulated inside the cells, while the formation of saposins and mature Cath D was impaired. Furthermore, neither Prosap nor proCath D were secreted from eHNPCs. The block of the processing and secretion shared by Prosap and proCath D was overcome during the course of differentiation of eHNPCs into a mixed population of astrocytes and neuronal cells. Upon differentiation, large amounts of Prosap and proCath D were secreted from the cells, while saposins and mature Cath D were produced inside the cells. The dramatic accumulation of Prosap (an antiapoptotic factor) and reduction of mature Cath D (a proapoptotic factor) in the undifferentiated eHNPCs most likely play a role in the molecular mechanisms regulating the resistance to apoptotic signals of these cells and might represent a critically important issue in HNPCs biology. © 2008 Elsevier B.V. All rights reserved.
Lingua originaleEnglish
pagine (da-a)1480-1489
Numero di pagine10
RivistaBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume1783
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Alternative Splicing
  • Alternative mRNA splicing
  • Biophysics
  • Cathepsin D
  • Cell Biology
  • Cell Differentiation
  • Cells, Cultured
  • Enzyme Precursors
  • Glycoside Hydrolases
  • Human neural stem cells
  • Humans
  • Molecular Biology
  • Neurons
  • Olfactory Bulb
  • Post-translational modifications
  • Procathepsin D
  • Prosaposin
  • Protein Precursors
  • Protein secretion
  • Saposins
  • Stem Cells

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