TY - JOUR
T1 - The role of molecular typing and perfect match transfusion in sickle cell disease and thalassaemia: An innovative transfusion strategy
AU - Putzulu, Rossana
AU - Piccirillo, Nicola
AU - Orlando, Nicoletta
AU - Massini, Giuseppina
AU - Maresca, Maddalena
AU - Scavone, Fernando
AU - Ricerca Storti, Bianca Maria
AU - Zini Tanzi, Gina
PY - 2017
Y1 - 2017
N2 - Chronic red blood cell transfusions remain an essential part of supportive treatment in patients with thalassaemia and sickle cell disease (SCD). Red blood cell (RBC) transfusions expose patients to the risk of developing antibodies: RBC alloimmunization occurs when the immune system meets foreign antigens. We created a register of extensively genotyped donors to achieve a better matched transfusion in order to reduce transfusion alloimmunization. Extended RBC antigen typing was determined and confirmed by molecular biology techniques using Human Erythrocyte Antigen (HEA) BeadChip (BioArray Solutions Ltd., Warren, NJ) in periodic blood donors and in patients with thalassaemia and SCD. During 3 years, we typed extensively 1220 periodic blood donors, 898 male and 322 female. We also studied 10 hematologic patients affected by thalassaemia and sickle cell disease referred to our institution as candidate to periodic transfusions. Our patients (8 females and 2 males with a median age of 48 years, range 24â76 years), extensively typed using molecular techniques and screened for RBC alloantibodies, were transfused with a median of 33.5 RBC units. After three years of molecular typing, the âperfect matchâ transfusion strategy avoided new alloantibodies development in all studied patients.
AB - Chronic red blood cell transfusions remain an essential part of supportive treatment in patients with thalassaemia and sickle cell disease (SCD). Red blood cell (RBC) transfusions expose patients to the risk of developing antibodies: RBC alloimmunization occurs when the immune system meets foreign antigens. We created a register of extensively genotyped donors to achieve a better matched transfusion in order to reduce transfusion alloimmunization. Extended RBC antigen typing was determined and confirmed by molecular biology techniques using Human Erythrocyte Antigen (HEA) BeadChip (BioArray Solutions Ltd., Warren, NJ) in periodic blood donors and in patients with thalassaemia and SCD. During 3 years, we typed extensively 1220 periodic blood donors, 898 male and 322 female. We also studied 10 hematologic patients affected by thalassaemia and sickle cell disease referred to our institution as candidate to periodic transfusions. Our patients (8 females and 2 males with a median age of 48 years, range 24â76 years), extensively typed using molecular techniques and screened for RBC alloantibodies, were transfused with a median of 33.5 RBC units. After three years of molecular typing, the âperfect matchâ transfusion strategy avoided new alloantibodies development in all studied patients.
KW - Alloimmunization
KW - Genotyping
KW - Hematology
KW - RBC antigens and antibodies
KW - Transfusion strategy
KW - Alloimmunization
KW - Genotyping
KW - Hematology
KW - RBC antigens and antibodies
KW - Transfusion strategy
UR - http://hdl.handle.net/10807/111695
UR - http://www.elsevier.com/locate/transci
U2 - 10.1016/j.transci.2017.01.003
DO - 10.1016/j.transci.2017.01.003
M3 - Article
SN - 1473-0502
VL - 56
SP - 234
EP - 237
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
ER -