TY - GEN
T1 - The role of caveolin-1 in the regulation of angiogenesis
AU - D'Alessio, Alessio
AU - Sica, Gigliola
PY - 2012
Y1 - 2012
N2 - Caveolin-1 (cav-1) is the principal structural component of caveolae which functions as scaffolding protein for the integration of a variety of signaling pathways. In this study, we show that siRNA-induced cav-1 down regulation in human endothelial cells (EC) increased cell size and provoked cell cycle arrest at G1/S phase transition. In addition, silencing of cav-1 reduced matrix metalloproteinases (MMPs) activity which, in turn, affected cell migration and VEGF-induced tube formation of EC in vitro. These data indicate that proper expression of cav-1 is required for maintaining typical functions of EC such as proliferation and the formation of new blood vessels. In addition, we observed a marked increase of cell size, after cav-1 silencing, which might indicate the involvement of this scaffolding protein in the way by which cells perceive changes in their microenvironment. In conclusion, this study proposes cav-1 as an interesting target molecule for studying cellular mechanisms which occur in physiological as well as pathological conditions such as senescence and tumorigenesis.
AB - Caveolin-1 (cav-1) is the principal structural component of caveolae which functions as scaffolding protein for the integration of a variety of signaling pathways. In this study, we show that siRNA-induced cav-1 down regulation in human endothelial cells (EC) increased cell size and provoked cell cycle arrest at G1/S phase transition. In addition, silencing of cav-1 reduced matrix metalloproteinases (MMPs) activity which, in turn, affected cell migration and VEGF-induced tube formation of EC in vitro. These data indicate that proper expression of cav-1 is required for maintaining typical functions of EC such as proliferation and the formation of new blood vessels. In addition, we observed a marked increase of cell size, after cav-1 silencing, which might indicate the involvement of this scaffolding protein in the way by which cells perceive changes in their microenvironment. In conclusion, this study proposes cav-1 as an interesting target molecule for studying cellular mechanisms which occur in physiological as well as pathological conditions such as senescence and tumorigenesis.
KW - Angiogenesis
KW - Caveolin
KW - Angiogenesis
KW - Caveolin
UR - http://hdl.handle.net/10807/18537
M3 - Conference contribution
T3 - ITALIAN JOURNAL OF ANATOMY AND EMBRYOLOGY
SP - 53
BT - 66° Congresso Nazionale S.I.A.I.
Società Italiana di Anatomia e Istologia Pistoia.
T2 - 66° Congresso Nazionale S.I.A.I.
Società Italiana di Anatomia e Istologia Pistoia.
Y2 - 20 September 2012 through 23 September 2012
ER -