The release of immunoreactive interleukin-1 beta from rat hypothalamic explants is modulated by neurotransmitters and corticotropin-releasing hormone

Giuseppe Tringali, Cesare Mancuso, Paolo Preziosi, Pierluigi Navarra, Giuseppe Guerriero, A Mirtella

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

Abstract

Both constitutive and inducible interleukin-1 (IL-1) gene expressions have been described in the central nervous system, the former being associated with immunoreactive IL-1 neurons in human and rat hypothalami. While most studies have focused on the role of IL-1 as a mediator of pathological events in the brain, the cytokine of neuronal origin might also be involved in the regulation of physiological processes. In this study we used a previously validated technique to investigate the effects of classical neurotransmitters and the hypophysiotropic peptide corticotropin-releasing hormone (CRH) on the release of immunoreactive (ir) IL-1 beta from acute rat hypothalamic explants. We found that basal irIL-1 beta secretion is significantly inhibited by acetylcholine and histamine and increased by dopamine, while dexamethasone, IL-4 and IL-10 have no effect. Moreover, cytokine release is dose-dependently increased by CRH. Such effects of the neurotransmitters and CRH are observed in short-term incubation experiments, indicating that a pre-formed pool of hypothalamic IL-1 beta is involved. These findings also suggest that the interaction between IL-1, neurotransmitters and neuropeptides might play a role in the physiological regulation of such processes as body temperature, food intake and the control of hypothalamic-hypophyseal axes.
Lingua originaleEnglish
pagine (da-a)269-273
Numero di pagine5
RivistaPharmacological Research
Volume36
Stato di pubblicazionePubblicato - 1997

Keywords

  • Acetylcholine
  • Animals
  • Corticotropin-Releasing Hormone
  • Dopamine
  • Histamine
  • Hypothalamus
  • Interleukin-1
  • Male
  • Neurotransmitter Agents
  • Rats
  • Rats, Wistar

Fingerprint Entra nei temi di ricerca di 'The release of immunoreactive interleukin-1 beta from rat hypothalamic explants is modulated by neurotransmitters and corticotropin-releasing hormone'. Insieme formano una fingerprint unica.

Cita questo