Abstract
In this paper, 10 mutations conditioning
the appearance of defective, miniature or collapsed endosperm, but with normal sporophyte development, were considered. Homozygous mutant kernels
have reduced grain weight, kernel size, density and,in some of these, higher than normal seed protein
content. The mutant loci were integrated into a highresolution
genetic map in order to associate them to specific genes. We have placed 1167 AFLP markers on a consensus map using IBM2 as a backbone and
reaching an average of 1 marker every 1.9 cM. We have identified AFLP markers linked to all individual
mutant alleles. BSA was adopted to screen the largest possible number of primer combinations on homozygous
F3 mutant and wild type plants. The ten
mutant loci are linked to the closest AFLP or SSR markers with distances ranging from 0 to 17.9 cM.
The genes we have defined by the existence of mendelian mutants can now be considered good candidates for testing the association to QT loci.
Lingua originale | English |
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pagine (da-a) | 527-541 |
Numero di pagine | 15 |
Rivista | Molecular Breeding |
Volume | 22 |
Stato di pubblicazione | Pubblicato - 2008 |
Keywords
- Zea mays
- bulked segregant analysis
- integrated molecular maps
- viable endosperm mutants