TY - JOUR
T1 - The immunosuppressive effect of human cytomegalovirus infection in recipients of allogeneic hematopoietic stem cell transplantation
AU - Giebel, S.
AU - Maccario, R.
AU - Lilleri, D.
AU - Zecca, M.
AU - Avanzini, M. A.
AU - Marconi, M.
AU - Di Cesare Merlone, A.
AU - Campanini, G.
AU - Montagna, D.
AU - Travaglino, P.
AU - Gentile, R.
AU - Gentile, Rocco
AU - Telli, S.
AU - Pagliara, D.
AU - Holowiecki, J.
AU - Locatelli, Franco
PY - 2005
Y1 - 2005
N2 - In immune-competent individuals, human cytomegalovirus ( HCMV) infection is associated with impairment of T-cell function. Our goal was to evaluate prospectively whether clinically asymptomatic HCMV infection in allogeneic hematopoietic stem cell transplantation (alloHSCT) recipients, treated pre emptively with ganciclovir, influences T-cell function as well. Mitogen-stimulated T-cell proliferative activity, together with cell surface markers, was tested in 49 patients on days +30, +45, +60, and +90 after alloHSCT and, additionally, in cases of positive HCMV pp65-antigenemia. HCMV infection was diagnosed in 19 patients. None of them developed HCMV disease. T-cell proliferative activity was significantly decreased on days when HCMV antigenemia was positive as compared to days without antigenemia. The number of pp65-positive cells negatively correlated with proliferative response. Comparison of patients who did experience HCMV infection with those who did not reveals significant decrease of T-cell proliferative activity observed on days +30 and +45, a time period when antigenemia was most frequently found to be positive, whereas no difference was detected on days +60 and +90. We conclude that, even clinically asymptomatic, HCMV infection has negative impact on T-cell proliferation capacity in alloHSCT recipients. However, pre emptive therapy with ganciclovir makes this immunosuppressive effect transient and restricted to the time of infection duration.
AB - In immune-competent individuals, human cytomegalovirus ( HCMV) infection is associated with impairment of T-cell function. Our goal was to evaluate prospectively whether clinically asymptomatic HCMV infection in allogeneic hematopoietic stem cell transplantation (alloHSCT) recipients, treated pre emptively with ganciclovir, influences T-cell function as well. Mitogen-stimulated T-cell proliferative activity, together with cell surface markers, was tested in 49 patients on days +30, +45, +60, and +90 after alloHSCT and, additionally, in cases of positive HCMV pp65-antigenemia. HCMV infection was diagnosed in 19 patients. None of them developed HCMV disease. T-cell proliferative activity was significantly decreased on days when HCMV antigenemia was positive as compared to days without antigenemia. The number of pp65-positive cells negatively correlated with proliferative response. Comparison of patients who did experience HCMV infection with those who did not reveals significant decrease of T-cell proliferative activity observed on days +30 and +45, a time period when antigenemia was most frequently found to be positive, whereas no difference was detected on days +60 and +90. We conclude that, even clinically asymptomatic, HCMV infection has negative impact on T-cell proliferation capacity in alloHSCT recipients. However, pre emptive therapy with ganciclovir makes this immunosuppressive effect transient and restricted to the time of infection duration.
KW - HCMV
KW - T cells
KW - alloHSCT
KW - HCMV
KW - T cells
KW - alloHSCT
UR - http://hdl.handle.net/10807/259145
U2 - 10.1038/sj.bmt.1705094
DO - 10.1038/sj.bmt.1705094
M3 - Article
SN - 0268-3369
VL - 36
SP - 503
EP - 509
JO - Bone Marrow Transplantation
JF - Bone Marrow Transplantation
ER -