The Enterococcus faecalis virulence factor ElrA interacts with the human Four-And-A-Half LIM Domains Protein

Francesca Bugli, Maurizio Sanguinetti, Brunella Posteraro, Alexandre Jamet, Rozenn Dervyn, Nicolas Lapaque, Naima G. Perez-Cortez, Hervé M. Blottière, Jean-Claude Twizere, Pascale Serror, Emmanuelle Maguin

Risultato della ricerca: Contributo in rivistaArticolo in rivista

3 Citazioni (Scopus)

Abstract

The commensal bacterium Enterococcus faecalis is a common cause of nosocomial infections worldwide. The increasing prevalence of multi-Antibiotic resistant E. faecalis strains reinforces this public health concern. Despite numerous studies highlighting several pathology-related genetic traits, the molecular mechanisms of E. faecalis virulence remain poorly understood. In this work, we studied 23 bacterial proteins that could be considered as virulence factors or involved in the Enterococcus interaction with the host. We systematically tested their interactions with human proteins using the Human ORFeome library, a set of 12,212 human ORFs, in yeast. Among the thousands of tested interactions, one involving the E. faecalis virulence factor ElrA and the human protein FHL2 was evidenced by yeast two-hybrid and biochemically confirmed. Further molecular characterizations allowed defining an FHL2-interacting domain (FID) of ElrA. Deletion of the FID led to an attenuated in vivo phenotype of the mutated strain clearly indicating that this interaction is likely to contribute to the multifactorial virulence of this opportunistic pathogen. Altogether, our results show that FHL2 is the first host cellular protein directly targeted by an E. faecalis virulence factor and that this interaction is involved in Enterococcus pathogenicity.
Lingua originaleEnglish
pagine (da-a)4581-N/A
RivistaScientific Reports
Volume7
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • Enterococcus

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