OBJECTIVE: To evaluate the effect of autologous platelet rich plasma (PRP)
combined with microfractures on the treatment of chondral defects. The hypothesis
of the study was that PRP can enhance cartilage repair after microfractures.
METHODS: A chronic full-thickness chondral lesion of the medial femoral condyle
was performed in 15 sheep. Animals were divided into three groups, according to
treatment: group 1: microfractures; group 2: microfractures+PRP and fibrin glue
gel; group 3: microfractures+liquid-PRP injection. Animals were sacrificed at 6
months after treatment. Macroscopic appearance was evaluated according to
International Cartilage Repair Society (ICRS) score; cartilage stiffness was
analyzed with an electromechanical indenter (Artscan 200); histological
appearance was scored according to a modified O'Driscoll score. Comparison
between groups for each outcome was performed with Kruskal-Wallis test, and
Tukey's test for pairwise comparisons.
RESULTS: Macroscopic ICRS score of group 2 was significantly better than those of
the other groups, and score of group 1 was significantly lower than those of the
other groups. Scores of group 1 and 3 were significantly lower than that of
normal cartilage. Mean cartilage stiffness of groups 1 and 3 was significantly
lower than that of normal cartilage. Histological total scores of group 2 and 3
were significantly better than that of group 1.
CONCLUSIONS: PRP showed a positive effect on cartilage repair and restoration
after microfractures. The procedure was more effective when PRP was used as a gel
in comparison with liquid intra-articular injection. Histological analysis
revealed that none of experimental treatments produced hyaline cartilage.
|Numero di pagine||10|
|Rivista||Osteoarthritis and Cartilage|
|Stato di pubblicazione||Pubblicato - 2010|
- Animal model
- Cartilage defect
- Cartilage repair
- Platelet rich plasma