The effect of oxygen transport resistances on the viability and functions of isolated rat hepatocytes

Celestino Pio Lombardi, G Catapano, L De Bartolo, E. Drioli

Risultato della ricerca: Contributo in rivistaArticolo in rivista

47 Citazioni (Scopus)

Abstract

The treatment of fulminant hepatic failure with a bioartificial liver support device relies on the possibility of replacing the detoxification and synthetic functions of the injured liver for as long as needed for patient recovery. In spite of progress in cell culture techniques, the effective use of isolated hepatocytes in liver support devices is currently hampered by a lack of information on the metabolic factors limiting long term hepatocyte culture. In this paper, we report our investigation on the effects of oxygen transport resistances on the viability and functions of isolated rat hepatocytes cultured on collagen coated Petri dishes. Detoxification and synthetic functions of the hepatocytes were studied with respect to ammonia and phenolsulphonphthalein elimination and urea synthesis. Lower resistances to oxygen transport favored hepatocyte survival. The isolated hepatocytes synthesized urea at rates that decreased as the resistance to oxygen transport increased. The rate at which urea was synthesized also decreased during the culture. Neither PSP, nor ammonia elimination rate was greatly affected by increasing oxygen transport resistances and remained rather constant up to a week of culture.
Lingua originaleEnglish
pagine (da-a)61-71
Numero di pagine11
RivistaInternational Journal of Artificial Organs
Volume19
Stato di pubblicazionePubblicato - 1996

Keywords

  • Ammonia
  • Animals
  • Artificial Organs
  • Biological Transport, Active
  • Cell Separation
  • Cell Survival
  • Cell Transplantation
  • Cells, Cultured
  • Hepatic Encephalopathy
  • Liver
  • Male
  • Mathematics
  • Microscopy, Electron, Scanning
  • Oxygen Consumption
  • Phenolsulfonphthalein
  • Rats
  • Rats, Wistar
  • Urea

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