The effect of catabolite concentration on the viability and functions of isolated rat hepatocytes

Celestino Pio Lombardi, G Catapano, L De Bartolo, E. Drioli

Risultato della ricerca: Contributo in rivistaArticolo in rivista

15 Citazioni (Scopus)

Abstract

The treatment of patients with hepatic failure by means of hybrid liver support devices using primary xenogeneic hepatocytes is currently hindered by the rapid loss of cell metabolic functions. Similarly to what happens with other mammalian cells, accumulation of catabolites in the neighborhood of cultured hepatocytes might significantly affect their viability and functions. In this paper, we investigated the effects of high concentrations of catabolites, such as ammonia and lactic acid, on the viability and functions of rat hepatocytes cultured on collagen coated Petri dishes. The effects on hepatocyte functions were established with respect to their ability to synthesize urea and to eliminate ammonia. Indeed, high catabolite concentrations effected both hepatocyte viability and functions. The number of viable hepatocytes decreased with increasing ammonia concentrations in the culture medium. High ammonia concentrations had also both an inhibitory and a toxic effect on hepatocyte functions. In fact, the hepatocytes synthesized urea and eliminated ammonia at rates that decreased with increasing ammonia concentrations. Similarly, high lactic acid concentrations were toxic to the cells and also inhibited their synthetic functions.
Lingua originaleEnglish
pagine (da-a)245-250
Numero di pagine6
RivistaInternational Journal of Artificial Organs
Volume19
Stato di pubblicazionePubblicato - 1996

Keywords

  • Ammonia
  • Animals
  • Cell Survival
  • Cells, Cultured
  • Collagen
  • Dose-Response Relationship, Drug
  • Glutamate Dehydrogenase
  • Lactates
  • Lactic Acid
  • Liver
  • Liver Regeneration
  • Male
  • Rats
  • Rats, Wistar
  • Urea

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