Target site mutations underlying insecticide resistance in Tunisian populations of Myzus persicae (Sulzer) on peach orchards and potato crops

Emanuele Mazzoni, Olga Chiesa, Amen Hlaoui, Christian C Figueroa, Rebha Souissi, Sonia Boukhris-Bouhachem

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

BACKGROUND: The massive use of synthetic insecticides strongly affects the level of insecticide resistance in populations of Myzus persicae worldwide. The selection of target site insensitivity-mutations is particularly worrying in areas where agro-industrial crops are vulnerable to the attacks of aphids that vector viruses, as in the case of Tunisia. Knowledge of the resistance mechanisms evolved locally in this aphid pest is a prerequisite to improving and retaining the sustainability of integrated pest management strategies. RESULTS: Target site mutations were surveyed in several populations of M. persicae collected from peach and potato crops between 2011 and 2017 in three Tunisian regions using real-time allele-specific PCR. The L1014F mutation (kdr locus) was found at a moderate frequency mostly in the heterozygous state and the homozygous resistant genotype was very uncommon. The M918T mutation (super-kdr locus) was present in a few heterozygous individuals, whereas the M918L mutation was detected for the first time in Tunisia and extreme North Africa. This latter mutation was shown to be widespread and well-established in Tunisia mainly as homozygous individuals, and was more abundant on peach than on potato crops. The S431F mutation (MACE) was found in a few heterozygous individuals. No individuals carrying the R81T mutation linked to neonicotinoid resistance were detected. CONCLUSION: This study points out a critical situation for the efficacy of pyrethroid insecticides to control M. persicae populations in Tunisia. It also confirms the rapid spread of the M918L mutation which has been detected in many different areas of the Mediterranean basin. © 2022 Society of Chemical Industry.
Lingua originaleEnglish
pagine (da-a)1594-1604
Numero di pagine11
RivistaPest Management Science
Volume78
DOI
Stato di pubblicazionePubblicato - 2022

Keywords

  • Insecticide resistance
  • Solanum tuberosum
  • L1014F
  • M918L
  • M918T
  • MACE
  • Myzus persicae
  • Qualitative Sybr green genotyping
  • R81T
  • Animals
  • Humans
  • Insecticide Resistance
  • Mutation
  • Aphids
  • Insecticides
  • Pyrethrins
  • kdr and super-kdr resistance

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