TY - JOUR
T1 - Synovial fluid-derived T helper 17 cells correlate with inflammatory activity in arthritis, irrespectively of diagnosis.
AU - Zizzo, Gaetano
AU - De Santis, Maria
AU - Bosello, Silvia Laura
AU - Fedele, Anna Laura
AU - Peluso, Giusy
AU - Gremese, Elisa
AU - Tolusso, Barbara
AU - Ferraccioli, Gianfranco
PY - 2011
Y1 - 2011
N2 - We analyzed peripheral blood (PB) and synovial fluid (SF) mononuclear cells from
16 rheumatoid arthritis (RA), 9 spondyloarthritis (SpA), 3 microcrystal arthritis
patients, to define the presence of Th17 and Th1 and their relationship with
inflammatory activity, and TCR-zeta chain and ZAP-70 levels. Th17 were
significantly higher in SF than in PB and more abundant in microcrystal arthritis
patients compared to the other groups. Irrespectively of the diagnosis, SF Th17
percentages correlated with joint (SF total leukocyte count, neutrophil
percentage) and systemic (C reactive protein [CRP], fibrinogen, erythrocyte
sedimentation rate) inflammation markers. SF Th1 percentages directly correlated
with inflammation and disease activity (CRP, swollen joint count [SJC]) indices
in SpA, but not in RA patients. These observations support the role of Th17 in
the pathogenesis of inflammatory arthritides. The TCR-zeta(dim) lymphocytes in SF
were found to produce the highest amounts of cytokines including IL-17, whereas
no ZAP-70 impairment was associated to Th17.
AB - We analyzed peripheral blood (PB) and synovial fluid (SF) mononuclear cells from
16 rheumatoid arthritis (RA), 9 spondyloarthritis (SpA), 3 microcrystal arthritis
patients, to define the presence of Th17 and Th1 and their relationship with
inflammatory activity, and TCR-zeta chain and ZAP-70 levels. Th17 were
significantly higher in SF than in PB and more abundant in microcrystal arthritis
patients compared to the other groups. Irrespectively of the diagnosis, SF Th17
percentages correlated with joint (SF total leukocyte count, neutrophil
percentage) and systemic (C reactive protein [CRP], fibrinogen, erythrocyte
sedimentation rate) inflammation markers. SF Th1 percentages directly correlated
with inflammation and disease activity (CRP, swollen joint count [SJC]) indices
in SpA, but not in RA patients. These observations support the role of Th17 in
the pathogenesis of inflammatory arthritides. The TCR-zeta(dim) lymphocytes in SF
were found to produce the highest amounts of cytokines including IL-17, whereas
no ZAP-70 impairment was associated to Th17.
KW - Rheumatoid Arthritis
KW - Th17
KW - synovial fluid
KW - Rheumatoid Arthritis
KW - Th17
KW - synovial fluid
UR - http://hdl.handle.net/10807/3403
U2 - 10.1016/j.clim.2010.10.002
DO - 10.1016/j.clim.2010.10.002
M3 - Article
SN - 1521-6616
VL - 138
SP - 107
EP - 116
JO - Clinical Immunology
JF - Clinical Immunology
ER -