Synovial B cells of rheumatoid arthritis express ZAP-70 which increases the survival and correlates with the inflammatory and autoimmune phenotype

Barbara Tolusso, Maria De Santis, Silvia Laura Bosello, Elisa Gremese, Stefania Gobessi, Ilaria Cuoghi, Michele C. Totaro, Giulio Bigotti, Carlo Rumi, Dimitar G. Efremov, Gianfranco Ferraccioli

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

B cells have acquired an important role in the pathogenesis of rheumatoid arthritis (PA) since B cell depletion allowed to rescue patients poorly responders to TNF alpha blockers. This study focused on the involvement of ZAP-70 as a bio-marker of B cells immune activation in RA. ZAP-70 expression in synovial fluid (SF) B cells obtained from RA patients was increased compared to SF B cells of osteoarthritis (OA) patients. Moreover we found that ZAP-70 positive/CD38 positive and ZAP-70 positive/CD5 positive B cells were enriched in SF. The analysis of B cell apoptosis in vitro showed that the percentage of ZAP-70 negative B cells spontaneously undergoing apoptosis was significantly higher than ZAP-70 positive B cells. The ZAP-70 positive B cell ratio (SF/peripheral blood (PB)) showed a positive correlation with SF autoantibody levels and with local levels of BAFF and IL6. ZAP-70 positive B cells seem to define a subset characterized by increased survival and high relationship with local inflammation and autoimmunity. (C) 2008 Elsevier Inc. All rights reserved.
Lingua originaleEnglish
pagine (da-a)98-108
Numero di pagine11
RivistaClinical Immunology
Volume131
DOI
Stato di pubblicazionePubblicato - 2009

Keywords

  • Rheumatoid arthritis
  • B cells
  • IL6
  • Synovial fluid
  • BAFF
  • ZAP-70

Fingerprint

Entra nei temi di ricerca di 'Synovial B cells of rheumatoid arthritis express ZAP-70 which increases the survival and correlates with the inflammatory and autoimmune phenotype'. Insieme formano una fingerprint unica.

Cita questo