Surface plasmon resonance: A useful strategy for the identification of small molecule argonaute 2 protein binders

Silvia Masciarelli, Elena Poser, Ilaria Genovese, Teresa Bellissimo, Francesco Fazi, Gianni Colotti

Risultato della ricerca: Contributo in libroChapter

3 Citazioni (Scopus)

Abstract

Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of light, is reflected when polarized light hits the film, monitored in real time as a change in the position of the dip in reflected intensity. Since SPR detects mass, the technique is label-free. Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening.
Lingua originaleEnglish
Titolo della pubblicazione ospiteDrug Target miRNA
Pagine223-237
Numero di pagine15
Volume1517
DOI
Stato di pubblicazionePubblicato - 2017

Serie di pubblicazioni

NomeMETHODS IN MOLECULAR BIOLOGY

Keywords

  • Analyte
  • Argonaute Proteins
  • High-Throughput Screening Assays
  • Humans
  • Ligand
  • Ligands
  • MicroRNAs
  • Molecular Docking Simulation
  • Protein Binding
  • Protein-inhibitor interaction
  • Small Molecule Libraries
  • Small molecules
  • Surface Plasmon Resonance
  • Surface plasmon resonance

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