@inbook{d4518af888eb4eb886c590a26c674287,
title = "Surface plasmon resonance: A useful strategy for the identification of small molecule argonaute 2 protein binders",
abstract = "Surface plasmon resonance (SPR) is one of the most important techniques for the detection and the characterization of molecular interactions. SPR technology is a label-free approach for monitoring biomolecular interactions in real time. The binding of analytes to molecules immobilized on a thin metal film (ligand) determines a change in the refractive index and, therefore in the angle of extinction of light, is reflected when polarized light hits the film, monitored in real time as a change in the position of the dip in reflected intensity. Since SPR detects mass, the technique is label-free. Here, we describe the use of SPR techniques to study the interaction between Argonaute 2 and small molecular compounds selected by means of high-throughput docking screening.",
keywords = "Analyte, Argonaute Proteins, High-Throughput Screening Assays, Humans, Ligand, Ligands, MicroRNAs, Molecular Docking Simulation, Protein Binding, Protein-inhibitor interaction, Small Molecule Libraries, Small molecules, Surface Plasmon Resonance, Surface plasmon resonance, Analyte, Argonaute Proteins, High-Throughput Screening Assays, Humans, Ligand, Ligands, MicroRNAs, Molecular Docking Simulation, Protein Binding, Protein-inhibitor interaction, Small Molecule Libraries, Small molecules, Surface Plasmon Resonance, Surface plasmon resonance",
author = "Elena Poser and Ilaria Genovese and Silvia Masciarelli and Teresa Bellissimo and Francesco Fazi and Gianni Colotti",
year = "2017",
doi = "10.1007/978-1-4939-6563-2_16",
language = "English",
isbn = "9781493982363",
volume = "1517",
series = "METHODS IN MOLECULAR BIOLOGY",
pages = "223--237",
booktitle = "Drug Target miRNA",
}