TY - JOUR
T1 - Study of cellular toxicity in vitro of two resins for orthodontic use
AU - Passarelli, Pier Carmine
AU - SACCOMANNO, S.
AU - De Angelis, Paolo
AU - ROMEO, A.
AU - PICCIRILLO, G. B.
AU - DESANTIS, V.
AU - Grippaudo, Cristina
AU - D'Addona, Antonio
PY - 2020
Y1 - 2020
N2 - Abstract. – OBJECTIVE: The objective of this work is to compare cellular toxicity in vitro of two resins for orthodontic use: an auto-polymerizable composite and a photo-polymerizable composite.\r\nMATERIALS AND METHODS: Samples were obtained by joining a couple of steel orthodontic brackets by using auto-polymerizing or photo-polymerizing resin. We used a halogen lamp, a mini LED lamp and a fast LED lamp used for orthodontics cure for 40 seconds. The 3T3 Swiss cellular line of fibroblasts was used. The samples obtained were used to determine the cellular toxicity in vitro using the Neutral Red Up-take (NRU) and the 3-(4,5-dimethylthiazol- 2-Yl)-2,5-diphenyltetrazolium bromide\r\n(MTT) assay.\r\nRESULTS: Toxicity of the extract appraised at a low level at MTT and NRU assays. There were statistically relevant differences between the toxicity induced by the auto-polymerizing material and the toxicity induced by the photo-polymerizing composite material, polymerized with the blue-light lamp (p < 0.001) and with the mini LED\r\nlamp (p < 0.05).\r\nCONCLUSIONS: From the data collected in this study, we can conclude that both resins show a low level of cytotoxicity that, in the case of photochemical polymerizing resin, depends on the characteristics of the lamp.
AB - Abstract. – OBJECTIVE: The objective of this work is to compare cellular toxicity in vitro of two resins for orthodontic use: an auto-polymerizable composite and a photo-polymerizable composite.\r\nMATERIALS AND METHODS: Samples were obtained by joining a couple of steel orthodontic brackets by using auto-polymerizing or photo-polymerizing resin. We used a halogen lamp, a mini LED lamp and a fast LED lamp used for orthodontics cure for 40 seconds. The 3T3 Swiss cellular line of fibroblasts was used. The samples obtained were used to determine the cellular toxicity in vitro using the Neutral Red Up-take (NRU) and the 3-(4,5-dimethylthiazol- 2-Yl)-2,5-diphenyltetrazolium bromide\r\n(MTT) assay.\r\nRESULTS: Toxicity of the extract appraised at a low level at MTT and NRU assays. There were statistically relevant differences between the toxicity induced by the auto-polymerizing material and the toxicity induced by the photo-polymerizing composite material, polymerized with the blue-light lamp (p < 0.001) and with the mini LED\r\nlamp (p < 0.05).\r\nCONCLUSIONS: From the data collected in this study, we can conclude that both resins show a low level of cytotoxicity that, in the case of photochemical polymerizing resin, depends on the characteristics of the lamp.
KW - citotoxicity
KW - composites
KW - citotoxicity
KW - composites
UR - https://publicatt.unicatt.it/handle/10807/146203
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85078941670&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85078941670&origin=inward
U2 - 10.26355/eurrev_202001_20078
DO - 10.26355/eurrev_202001_20078
M3 - Article
SN - 2284-0729
VL - 24
SP - 930
EP - 934
JO - European Review for Medical and Pharmacological Sciences
JF - European Review for Medical and Pharmacological Sciences
IS - 2
ER -