Structural and functional studies on a proline-rich peptide isolated from swine saliva endowed with antifungal activity towards Cryptococcus neoformans.

Massimo Castagnola, Stefania Conti, Tecla Ciociola, Renato Longhi, Luciano Polonelli, Rita Gatti, Tiziana Cabras, Irene Messana, Alberto Vitali

Risultato della ricerca: Contributo in rivistaArticolo in rivista

17 Citazioni (Scopus)

Abstract

A proline-rich peptide of 2733Da, isolated from pig parotid granule preparations was tested against different pathogenic fungi. It showed interesting antifungal activity towards a clinical isolate of Cryptococcus neoformans, with an EC(50) of 2.2μM. Neither cytotoxic nor haemolytic effects were observed towards mammalian cells. Circular dichroism and infrared spectroscopic studies showed that the peptide adopted a combination of polyproline type-II, β-turn and unordered conformations at physiological temperatures. Temperature dependent experiments evidenced a tendency to adopt a polyproline-II helix conformation. From experiments with lipid vesicles, Neutral Red Uptake (NRU), haemolytic assays, and confocal microscopy studies, it could be hypothesized that the peptide may exert its antifungal effect by interacting with an intracellular target rather than through membrane damage.
Lingua originaleEnglish
pagine (da-a)1066-1074
Numero di pagine9
RivistaBIOCHIMICA ET BIOPHYSICA ACTA
Volume1828
Stato di pubblicazionePubblicato - 2013

Keywords

  • PEPTIDE
  • SALIVA

Fingerprint Entra nei temi di ricerca di 'Structural and functional studies on a proline-rich peptide isolated from swine saliva endowed with antifungal activity towards Cryptococcus neoformans.'. Insieme formano una fingerprint unica.

Cita questo