Somatic cell nuclear transfer and transgenesis in large animals: current and future insights

Franco Lucchini, C. Galli, I. Lagutina, A. Perota, S. Colleoni, R. Duchi, G. Lazzari

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

35 Citazioni (Scopus)

Abstract

Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well-established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target-specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.
Lingua originaleEnglish
pagine (da-a)2-11
Numero di pagine10
RivistaReproduction in Domestic Animals
Volume47 Suppl 3
DOI
Stato di pubblicazionePubblicato - 2012

Keywords

  • Transgenic animal
  • Zinc finger nuclease
  • short-interfering RNA (siRNA)
  • somatic cell nuclear transfer (SCNT)

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