TY - JOUR
T1 - Somatic cell nuclear transfer and transgenesis in large animals: current and future insights
AU - Galli, C.
AU - Lagutina, I.
AU - Perota, A.
AU - Colleoni, S.
AU - Duchi, R.
AU - Lucchini, Franco
AU - Lazzari, G.
PY - 2012
Y1 - 2012
N2 - Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well-established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target-specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.
AB - Somatic cell nuclear transfer (SCNT) was first developed in livestock for the purpose of accelerating the widespread use of superior genotypes. Although many problems still exist now after fifteen years of research owing to the limited understanding of genome reprogramming, SCNT has provided a powerful tool to make copies of selected individuals in different species, to study genome pluripotency and differentiation, opening new avenues of research in regenerative medicine and representing the main route for making transgenic livestock. Besides well-established methods to deliver transgenes, recent development in enzymatic engineering to edit the genome provides more precise and reproducible tools to target-specific genomic loci especially for producing knockout animals. The interest in generating transgenic livestock lies in the agricultural and biomedical areas and it is, in most cases, at the stage of research and development, with few exceptions that are making the way into practical applications.
KW - Transgenic animal
KW - Zinc finger nuclease
KW - short-interfering RNA (siRNA)
KW - somatic cell nuclear transfer (SCNT)
KW - Transgenic animal
KW - Zinc finger nuclease
KW - short-interfering RNA (siRNA)
KW - somatic cell nuclear transfer (SCNT)
UR - http://hdl.handle.net/10807/23190
UR - http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0531.2012.02045.x/pdf
U2 - 10.1111/j.1439-0531.2012.02045.x
DO - 10.1111/j.1439-0531.2012.02045.x
M3 - Article
SN - 0936-6768
VL - 47 Suppl 3
SP - 2
EP - 11
JO - Reproduction in Domestic Animals
JF - Reproduction in Domestic Animals
ER -