Small molecules targeting the miRNA-binding domain of argonaute 2: From computer-aided molecular design to RNA immunoprecipitation

Silvia Masciarelli, Teresa Bellissimo, Elena Poser, Ilaria Genovese, Alberto Del Rio, Gianni Colotti, Francesco Fazi

Risultato della ricerca: Contributo in libroChapter

2 Citazioni (Scopus)

Abstract

The development of small-molecule-based target therapy design for human disease and cancer is object of growing attention. Recently, specific microRNA (miRNA) mimicking compounds able to bind the miRNA-binding domain of Argonaute 2 protein (AGO2) to inhibit miRNA loading and its functional activity were described. Computer-aided molecular design techniques and RNA immunoprecipitation represent suitable approaches to identify and experimentally determine if a compound is able to impair the loading of miRNAs on AGO2 protein. Here, we describe these two methodologies that we recently used to select a specific compound able to interfere with the AGO2 functional activity and able to improve the retinoic acid-dependent myeloid differentiation of leukemic cells.
Lingua originaleEnglish
Titolo della pubblicazione ospiteMethods in Molecular Biology
Pagine211-221
Numero di pagine11
Volume1517
DOI
Stato di pubblicazionePubblicato - 2017

Keywords

  • Argonaute 2
  • Argonaute Proteins
  • Cell Differentiation
  • Drug Delivery Systems
  • Gene Expression Regulation, Leukemic
  • Humans
  • Immunoprecipitation
  • Leukemia
  • MicroRNAs
  • Models, Molecular
  • Molecular docking techniques
  • Myeloid differentiation
  • RNA immunoprecipitation
  • Small Molecule Libraries
  • Small molecules
  • Tretinoin

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