SlyA is a transcriptional regulator involved in the virulence of Enterococcus faecalis

Maurizio Sanguinetti, Brunella Posteraro, Charlotte Michaux, Fany Reffuveille, Yanick Auffray, Michael S. Gilmore, Axel Hartke, Jean-Christophe Giard

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

42 Citazioni (Scopus)

Abstract

Phylogenetic analysis of the crystal structure of the Enterococcus faecalis SlyA (EF_3002) transcriptional factor places it between the SlyA and MarR regulator subfamilies. Proteins of these families are often involved in the regulation of genes important for bacterial virulence and stress response. To gather evidence for the role of this putative regulator in E. faecalis biology, we dissected the genetic organization of the slyA-EF_3001 locus and constructed a slyA deletion mutant as well as complemented strains. Interestingly, compared to the wild-type parent, the ΔslyA mutant is more virulent in an insect infection model (Galleria mellonella), exhibits increased persistence in mouse kidneys and liver, and survives better inside peritoneal macrophages. In order to identify a possible SlyA regulon, global microarray transcriptional analysis was performed. This study revealed that the slyA-EF_3001 locus appears to be autoregulated and that 117 genes were differentially regulated in the ΔslyA mutant. In the mutant strain, 111 were underexpressed and 6 overexpressed, indicating that SlyA functions mainly as an activator of transcription.
Lingua originaleEnglish
pagine (da-a)2638-2645
Numero di pagine8
RivistaInfection and Immunity
Volume79
DOI
Stato di pubblicazionePubblicato - 2011

Keywords

  • Animals
  • Bacterial Proteins
  • Base Sequence
  • Enterococcus faecalis
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Larva
  • Microarray Analysis
  • Moths
  • Phylogeny
  • Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Transcription Factors
  • Transcription, Genetic

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