Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Tiziana D'Inzeo; Giulia De Angelis; Maurizio Sanguinetti; Brunella Posteraro; Teresa Spanu; Liliana Giordano; Barbara Fiori Alfarano; Flora Marzia Liotti; Giulia Menchinelli; Flavio De Maio; Gabriella Parisi; Francesco Luzzaro

doi:10.1128/AAC.00550-19

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Tiziana D'Inzeo, Giulia De Angelis, Maurizio Sanguinetti, Brunella Posteraro, Teresa Spanu, Liliana Giordano, Barbara Fiori Alfarano, Flora Marzia Liotti, Giulia Menchinelli, Flavio De Maio, Gabriella Parisi, Francesco Luzzaro

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

12 Citazioni (Scopus)

Abstract

We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.

Lingua originale	English
pagine (da-a)	N/A-N/A
Rivista	Antimicrobial Agents and Chemotherapy
Volume	63
DOI	https://doi.org/10.1128/AAC.00550-19
Stato di pubblicazione	Pubblicato - 2019

Keywords

Carbapenem resistance
Enterobacterales
Immunochromatographic assay
Positive blood culture

Accesso al documento

10.1128/AAC.00550-19

Altri file e collegamenti

Cita questo

D'Inzeo, T., De Angelis, G., Sanguinetti, M., Posteraro, B., Spanu, T., Giordano, L., Fiori Alfarano, B., Liotti, F. M., Menchinelli, G., De Maio, F., Parisi, G., & Luzzaro, F. (2019). Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay. Antimicrobial Agents and Chemotherapy, 63, N/A-N/A. https://doi.org/10.1128/AAC.00550-19

@article{f5dfc556affe45ababe0dc836add0b1a,

title = "Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay",

abstract = "We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.",

keywords = "Carbapenem resistance, Enterobacterales, Immunochromatographic assay, Positive blood culture, Carbapenem resistance, Enterobacterales, Immunochromatographic assay, Positive blood culture",

author = "Tiziana D'Inzeo and {De Angelis}, Giulia and Maurizio Sanguinetti and Brunella Posteraro and Teresa Spanu and Liliana Giordano and {Fiori Alfarano}, Barbara and Liotti, {Flora Marzia} and Giulia Menchinelli and {De Maio}, Flavio and Gabriella Parisi and Francesco Luzzaro",

year = "2019",

doi = "10.1128/AAC.00550-19",

language = "English",

volume = "63",

pages = "N/A--N/A",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology:PO Box 11127:Birmingham, AL 35201:(800)633-4931, (205)995-1567, EMAIL: asm.nonmembers.subs@ebsco.com, INTERNET: http://www.asm.org, Fax: (205)995-1588",

}

D'Inzeo, T , De Angelis, G , Sanguinetti, M , Posteraro, B , Spanu, T, Giordano, L, Fiori Alfarano, B, Liotti, FM, Menchinelli, G, De Maio, F, Parisi, G & Luzzaro, F 2019, 'Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay', Antimicrobial Agents and Chemotherapy, vol. 63, pagg. N/A-N/A. https://doi.org/10.1128/AAC.00550-19

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay. / D'Inzeo, Tiziana ; De Angelis, Giulia ; Sanguinetti, Maurizio et al.

In: Antimicrobial Agents and Chemotherapy, Vol. 63, 2019, pag. N/A-N/A.

Risultato della ricerca: Contributo in rivista › Articolo in rivista › peer review

TY - JOUR

T1 - Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

AU - D'Inzeo, Tiziana

AU - De Angelis, Giulia

AU - Sanguinetti, Maurizio

AU - Posteraro, Brunella

AU - Spanu, Teresa

AU - Giordano, Liliana

AU - Fiori Alfarano, Barbara

AU - Liotti, Flora Marzia

AU - Menchinelli, Giulia

AU - De Maio, Flavio

AU - Parisi, Gabriella

AU - Luzzaro, Francesco

PY - 2019

Y1 - 2019

N2 - We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.

AB - We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.

KW - Carbapenem resistance

KW - Enterobacterales

KW - Immunochromatographic assay

KW - Positive blood culture

KW - Carbapenem resistance

KW - Enterobacterales

KW - Immunochromatographic assay

KW - Positive blood culture

UR - http://hdl.handle.net/10807/139382

UR - https://aac.asm.org/content/aac/63/7/e00550-19.full.pdf

U2 - 10.1128/AAC.00550-19

DO - 10.1128/AAC.00550-19

M3 - Article

VL - 63

SP - N/A-N/A

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

ER -

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Abstract

Keywords

Accesso al documento

Altri file e collegamenti

Fingerprint

Cita questo