TY - JOUR
T1 - Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay
AU - Giordano, Liliana
AU - Fiori, Barbara
AU - D'Inzeo, Tiziana
AU - Parisi, Gabriella
AU - Liotti, Flora Marzia
AU - Menchinelli, Giulia
AU - De Angelis, Giulia
AU - De Maio, Flavio
AU - Luzzaro, Francesco
AU - Sanguinetti, Maurizio
AU - Posteraro, Brunella
AU - Spanu Pennestri, Teresa
PY - 2019
Y1 - 2019
N2 - We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
AB - We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
KW - Carbapenem resistance
KW - Enterobacterales
KW - Immunochromatographic assay
KW - Positive blood culture
KW - Carbapenem resistance
KW - Enterobacterales
KW - Immunochromatographic assay
KW - Positive blood culture
UR - http://hdl.handle.net/10807/139382
UR - https://aac.asm.org/content/aac/63/7/e00550-19.full.pdf
U2 - 10.1128/AAC.00550-19
DO - 10.1128/AAC.00550-19
M3 - Article
SN - 0066-4804
VL - 63
SP - N/A-N/A
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
ER -