TY - JOUR
T1 - Short tandem repeat profiling for the authentication of cancer stem-like cells
AU - Visconti, Paola
AU - Parodi, Federica
AU - Parodi, Barbara
AU - Casarino, Lucia
AU - Romano, Paolo
AU - Buccarelli, Mariachiara
AU - Pallini, Roberto
AU - D'Alessandris, Quintino Giorgio
AU - Montori, Andrea
AU - Pilozzi, Emanuela
AU - Ricci-Vitiani, Lucia
PY - 2021
Y1 - 2021
N2 - Colorectal and glioblastoma cancer stem-like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross-contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.
AB - Colorectal and glioblastoma cancer stem-like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross-contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.
KW - cell line authentication
KW - colorectal tumor
KW - glioblastoma
KW - human stem-like cell lines
KW - short tandem repeat profiling
KW - cell line authentication
KW - colorectal tumor
KW - glioblastoma
KW - human stem-like cell lines
KW - short tandem repeat profiling
UR - http://hdl.handle.net/10807/218995
U2 - 10.1002/ijc.33370
DO - 10.1002/ijc.33370
M3 - Article
SN - 0020-7136
VL - 148
SP - 1489
EP - 1498
JO - International Journal of Cancer
JF - International Journal of Cancer
ER -