SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Roberto Scatena, Barbara Tavazzi, Giuseppina Nocca, Massimo Castagnola, Diana Valeria Rossetti, Bruno Giardina, D Dipierro

Risultato della ricerca: Contributo in rivistaArticolo in rivista

7 Citazioni (Scopus)

Abstract

The separation of reduced and oxidized glutathione at an absolute sensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high efficiency and good reliability. A partition mechanism is responsible for the high resolution observed. The method was utilized for the analysis of commercial preparations of glutathione and a good agreement with the expected results was obtained; the oxidation of the commercial glutathione in solution was easily analysed.
Lingua originaleEnglish
pagine (da-a)220-226
Numero di pagine7
RivistaBiomedical Chromatography
Volume7
DOI
Stato di pubblicazionePubblicato - 1993

Keywords

  • CAPILLARY

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