SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Massimo Castagnola; D Dipierro; Roberto Scatena; Barbara Tavazzi; Giuseppina Nocca; Diana Valeria Rossetti; Bruno Giardina

doi:10.1002/bmc.1130070410

SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Massimo Castagnola, D Dipierro, Roberto Scatena, Barbara Tavazzi, Giuseppina Nocca, Diana Valeria Rossetti, Bruno Giardina

Risultato della ricerca: Contributo in rivista › Articolo in rivista

7 Citazioni (Scopus)

Abstract

The separation of reduced and oxidized glutathione at an absolute sensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high efficiency and good reliability. A partition mechanism is responsible for the high resolution observed. The method was utilized for the analysis of commercial preparations of glutathione and a good agreement with the expected results was obtained; the oxidation of the commercial glutathione in solution was easily analysed.

Lingua originale	English
pagine (da-a)	220-226
Numero di pagine	7
Rivista	Biomedical Chromatography
Volume	7
DOI	https://doi.org/10.1002/bmc.1130070410
Stato di pubblicazione	Pubblicato - 1993

Keywords

CAPILLARY

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10.1002/bmc.1130070410

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title = "SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY",

abstract = "The separation of reduced and oxidized glutathione at an absolute sensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high efficiency and good reliability. A partition mechanism is responsible for the high resolution observed. The method was utilized for the analysis of commercial preparations of glutathione and a good agreement with the expected results was obtained; the oxidation of the commercial glutathione in solution was easily analysed.",

keywords = "CAPILLARY, CAPILLARY",

author = "Massimo Castagnola and D Dipierro and Roberto Scatena and Barbara Tavazzi and Giuseppina Nocca and Rossetti, {Diana Valeria} and Bruno Giardina",

year = "1993",

doi = "10.1002/bmc.1130070410",

language = "English",

volume = "7",

pages = "220--226",

journal = "Biomedical Chromatography",

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TY - JOUR

T1 - SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

AU - Castagnola, Massimo

AU - Dipierro, D

AU - Scatena, Roberto

AU - Tavazzi, Barbara

AU - Nocca, Giuseppina

AU - Rossetti, Diana Valeria

AU - Giardina, Bruno

PY - 1993

Y1 - 1993

N2 - The separation of reduced and oxidized glutathione at an absolute sensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high efficiency and good reliability. A partition mechanism is responsible for the high resolution observed. The method was utilized for the analysis of commercial preparations of glutathione and a good agreement with the expected results was obtained; the oxidation of the commercial glutathione in solution was easily analysed.

AB - The separation of reduced and oxidized glutathione at an absolute sensitivity of about 100 pg by micellar electrokinetic capillary chromatography without derivatization is described. The time required for the separation is less than 10 min (the time between two following injections is about 15 min). The separation is characterized by high efficiency and good reliability. A partition mechanism is responsible for the high resolution observed. The method was utilized for the analysis of commercial preparations of glutathione and a good agreement with the expected results was obtained; the oxidation of the commercial glutathione in solution was easily analysed.

KW - CAPILLARY

UR - http://hdl.handle.net/10807/23605

U2 - 10.1002/bmc.1130070410

DO - 10.1002/bmc.1130070410

M3 - Article

SN - 0269-3879

VL - 7

SP - 220

EP - 226

JO - Biomedical Chromatography

JF - Biomedical Chromatography

ER -

SEPARATION OF REDUCED AND OXIDIZED GLUTATHIONE BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Abstract

Keywords

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