SEMA6C: a novel adhesion-independent FAK and YAP activator, required for cancer cell viability and growth

Damon Fard, Erika Testa, Valentina Panzeri, Sabrina Rizzolio, Giada Bianchetti, Virginia Napolitano, Silvia Masciarelli, Francesco Fazi, Giuseppe Maulucci, Bianca Maria Scicchitano, Claudio Sette, Maria Teresa Viscomi, Luca Tamagnone*

*Autore corrispondente per questo lavoro

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Transmembrane semaphorins are signaling molecules, controlling axonal wiring and embryo development, which are increasingly implicated in human diseases. Semaphorin 6C (Sema6C) is a poorly understood family member and its functional role is still unclear. Upon targeting Sema6C expression in a range of cancer cells, we observed dramatic growth suppression, decreased ERK phosphorylation, upregulation of cell cycle inhibitor proteins p21, p27 and p53, and the onset of cell senescence, associated with activation of autophagy. These data are consistent with a fundamental requirement for Sema6C to support viability and growth in cancer cells. Mechanistically, we unveiled a novel signaling pathway elicited by Sema6C, and dependent on its intracellular domain, mediated by tyrosine kinases c-Abl and Focal Adhesion Kinase (FAK). Sema6C was found in complex with c-Abl, and induced its phosphorylation, which in turn led to FAK activation, independent of cell-matrix adhesion. Sema6C-induced FAK activity was furthermore responsible for increased nuclear localization of YAP transcriptional regulator. Moreover, Sema6C conferred YAP signaling-dependent long-term cancer cell survival upon nutrient deprivation. In conclusion, our findings demonstrate that Sema6C elicits a cancer promoting-signaling pathway sustaining cell viability and self-renewal, independent of growth factors and nutrients availability.
Lingua originaleEnglish
pagine (da-a)1-15
Numero di pagine15
RivistaCellular and Molecular Life Sciences
Volume2023
DOI
Stato di pubblicazionePubblicato - 2023

Keywords

  • FAK
  • KINASE
  • SEMAPHORIN
  • SIGNALING PATHWAY
  • YAP

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