SAP-mediated inhibition of diacylglycerol kinase α regulates TCR-induced diacylglycerol signaling

Ornella Parolini, Gianluca Baldanzi, Andrea Pighini, Valentina Bettio, Elena Rainero, Sara Traini, Federica Chianale, Paolo E. Porporato, Nicoletta Filigheddu, Riccardo Mesturini, Shuping Song, Tamas Schweighoffer, Laura Patrussi, Cosima T. Baldari, Xiao-Ping Zhong, Wim J. Van Blitterswijk, Fabiola Sinigaglia, Kim E. Nichols, Ignacio Rubio, Andrea Graziani

Risultato della ricerca: Contributo in rivistaArticolo in rivista

34 Citazioni (Scopus)

Abstract

Diacylglycerol kinases (DGKs) metabolize diacylglycerol to phosphatidic acid. In T lymphocytes, DGKα acts as a negative regulator of TCR signaling by decreasing diacylglycerol levels and inducing anergy. In this study, we show that upon costimulation of the TCR with CD28 or signaling lymphocyte activation molecule (SLAM), DGKα, but not DGKζ, exits from the nucleus and undergoes rapid negative regulation of its enzymatic activity. Inhibition of DGKα is dependent on the expression of SAP, an adaptor protein mutated in X-linked lymphoproliferative disease, which is essential for SLAM-mediated signaling and contributes to TCR/CD28-induced signaling and T cell activation. Accordingly, overexpression of SAP is sufficient to inhibit DGKα, whereas SAP mutants unable to bind either phospho-tyrosine residues or SH3 domain are ineffective. Moreover, phospholipase C activity and calcium, but not Src-family tyrosine kinases, are also required for negative regulation of DGKα. Finally, inhibition of DGKα in SAP-deficient cells partially rescues defective TCR/CD28 signaling, including Ras and ERK1/2 activation, protein kinase C membrane recruitment, induction of NF-AT transcriptional activity, and IL-2 production. Thus SAP-mediated inhibition of DGKα sustains diacylglycerol signaling, thereby regulating T cell activation, and it may represent a novel pharmacological strategy for X-linked lymphoproliferative disease treatment.
Lingua originaleEnglish
pagine (da-a)5941-5951
Numero di pagine11
RivistaJOURNAL OF IMMUNOLOGY
Volume187
DOI
Stato di pubblicazionePubblicato - 2011

Keywords

  • Blotting, Western
  • Diacylglycerol Kinase
  • Diglycerides
  • Fluorescent Antibody Technique
  • Humans
  • Immunoprecipitation
  • Intracellular Signaling Peptides and Proteins
  • Jurkat Cells
  • Lymphocyte Activation
  • Protein Transport
  • Receptors, Antigen, T-Cell
  • Signal Transduction
  • T-Lymphocytes
  • Transfection

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