S. Aureus biofilm protein expression linked to antimicrobial resistance: A` proteomic study

Viviana Greco, Andrea Urbani, Cristian Piras, Pierluigi Aldo Di Ciccio, Alessio Soggiu, Bruno Tilocca, Nicola Costanzo, Carlotta Ceniti, Luigi Bonizzi, Adriana Ianieri, Paola Roncada

Risultato della ricerca: Contributo in rivistaArticolo in rivista

Abstract

Antimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a "One Health" approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the spread of AMR. One of the most common ways for bacteria to live is to adhere to surfaces and form biofilms. Staphylococcus aureus (S. aureus) can form biofilm on most surfaces and in a wide heterogeneity of environmental conditions. The biofilm guarantees the survival of the S. aureus in harsh environmental conditions and represents an issue for the food industry and animal production. The identification and characterization of biofilm-related proteins may provide interesting insights into biofilm formation mechanisms in S. aureus. In this regard, the aims of this study were: (i) to use proteomics to compare proteomes of S. aureus growing in planktonic and biofilm forms in order to investigate the common features of biofilm formation properties of different strains; (ii) to identify specific biofilm mechanisms that may be involved in AMR. The proteomic analysis showed 14 differentially expressed proteins among biofilm and planktonic forms of S. aureus. Moreover, three proteins, such as alcohol dehydrogenase, ATP-dependent 6-phosphofructokinase, and fructose-bisphosphate aldolase, were only differentially expressed in strains classified as high biofilm producers. Differentially regulated catabolites metabolisms and the switch to lower oxygen-related metabolisms were related to the sessile conformation analyzed.
Lingua originaleEnglish
pagine (da-a)1-12
Numero di pagine12
RivistaAnimals
DOI
Stato di pubblicazionePubblicato - 2021

Keywords

  • proteomics

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