Rotating-disc micro-solid phase extraction of F2-isoprostanes from maternal and cord plasma by using oxidized buckypaper as sorbent membrane

Luciana Teofili, Patrizia Papacci, Giovanni Vento, Velia Purcaro, Maria Sofia Cori, Pierpaolo Tomai, Andrea Martinelli, Tecla Gasperi, Roberta Curini, Salvatore Fanali, Alessandra Gentili

Risultato della ricerca: Contributo in rivistaArticolo in rivistapeer review

6 Citazioni (Scopus)

Abstract

This paper describes the development of an original micro-solid phase extraction device and its evaluation for the isolation of F2-isoprostanes (F2-IsoPs) from cord and maternal plasma samples. The unit is very simple and consists in a rotating disc (1.8 cm diameter) of oxidized buckypaper (BP), enwrapped in a polypropylene mesh pouch. Even if the selected F2-IsoPs have logP and pKa values that make them suitable candidates for their sorption on BP, several parameters were optimized to maximize recoveries: time of adsorption and desorption; stirring speed; volume, pH and ionic strength of the sample; type, volume, and fractions of the elution solvent; oxidation grade of BP. Among all, the last one was crucial in affecting extraction yields because of the analyte interactions with polar functionalities, introduced by a preliminary oxidative acid treatment. The investigation established the optimal oxidation time and highlighted the pros and cons of the acid activation step. All extracts were analyzed by means of liquid chromatography-tandem mass spectrometry (LC–MS/MS). Validation was performed according to the main FDA guidelines for bioanalytical methods. Depending on the spike level and analyte, recoveries ranged between 30 and 120% with precision and accuracy values lower than 20%. Quantitative analysis was accomplished by matrix-matched calibration curves whose determination coefficients were higher than 0.95. Lower limit of quantitation (LLOQ) spanned the range 2.45–6.77 μg L−1. The validated method was applied to the analysis of eight pairs of mother/child plasma samples, revealing the presence of 8-iso-15-keto-PGF2α and 8-iso-PGE2 at a concentration of about 10 μg L−1 in most cord plasma samples of preterm newborns.
Lingua originaleEnglish
pagine (da-a)30-39
Numero di pagine10
RivistaJOURNAL OF CHROMATOGRAPHY A
Volume1586
DOI
Stato di pubblicazionePubblicato - 2019

Keywords

  • Adsorption
  • Biological samples
  • Buckypaper
  • Carbon nanotubes
  • F2-Isoprostanes
  • Female
  • Fetal Blood
  • Humans
  • Infant, Newborn
  • LC–MS
  • Limit of Detection
  • Nanotubes, Carbon
  • Paper
  • Pregnancy
  • Sample preparation
  • Solid Phase Extraction
  • Solid phase extraction
  • Solvents
  • Tandem Mass Spectrometry

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