Roscovitine sensitizes breast cancer cells to TRAIL-induced apoptosis through a pleiotropic mechanism

Ruggero De Maria Marchiano, Adriana Eramo, Gustavo Ortiz-Ferrón, Rosario Yerbes, Ana I López-Pérez, Abelardo López-Rivas

Risultato della ricerca: Contributo in rivistaArticolo in rivista

31 Citazioni (Scopus)

Abstract

The tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL/APO2L) is a member of the TNF gene superfamily that induces apoptosis upon engagement of cognate death receptors. While TRAIL is relatively non-toxic to normal cells, it selectively induces apoptosis in many transformed cells. Nevertheless, breast tumor cells are particularly resistant to the effects of TRAIL. Here we report that, in combination with the cyclin-dependent kinase inhibitor roscovitine, exposure to TRAIL induced marked apoptosis in the majority of TRAIL-resistant breast cancer cell lines examined. Roscovitine facilitated TRAIL death-inducing signaling complex formation and the activation of caspase-8. The cFLIP L and cFLIP S FLICE-inhibitory proteins were significantly down-regulated following exposure to roscovitine and, indeed, the knockdown of cFLIP isoforms by siRNA sensitized breast tumor cells to TRAIL-induced apoptosis. In addition, we demonstrate that roscovitine strongly suppressed Mcl-1 expression and up-regulated E2F1 protein levels in breast tumor cells. Significantly, the silencing of Mcl-1 by siRNA sensitized breast tumor cells to TRAIL-induced apoptosis. Furthermore, the knockdown of E2F1 protein by siRNA reduced the sensitizing effect of roscovitine in TRAIL-induced apoptosis. In summary, our results reveal a pleitropic mechanism for the pro-apoptotic influence of roscovitine, highlighting its potential as an antitumor agent in breast cancer in combination with TRAIL. © 2008 IBCB, SIBS, CAS All rights reserved.
Lingua originaleEnglish
pagine (da-a)664-676
Numero di pagine13
RivistaCell Research
Volume18
DOI
Stato di pubblicazionePubblicato - 2008

Keywords

  • Antineoplastic Agents
  • Apoptosis
  • Breast Neoplasms
  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • CDK
  • Caspases
  • Cell Biology
  • Cell Line, Tumor
  • Cell Membrane
  • DISC
  • Death Domain Receptor Signaling Adaptor Proteins
  • Down-Regulation
  • Drug Screening Assays, Antitumor
  • E2F1
  • E2F1 Transcription Factor
  • Enzyme Activation
  • FLIP
  • Fas-Associated Death Domain Protein
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Mcl-1
  • Membrane Microdomains
  • Molecular Biology
  • Myeloid Cell Leukemia Sequence 1 Protein
  • Protein Isoforms
  • Proto-Oncogene Proteins c-bcl-2
  • Purines
  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • Roscovitine
  • TNF-Related Apoptosis-Inducing Ligand
  • TRAIL

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