TY - JOUR
T1 - Role of tissue C-reactive protein in atrial cardiomyocytes of patients undergoing catheter ablation of atrial fibrillation: Pathogenetic implications
AU - Narducci, Maria Lucia
AU - Pelargonio, Gemma
AU - Dello Russo, Antonio
AU - Casella, Michela
AU - Biasucci, Luigi Marzio
AU - La Torre, Giuseppe
AU - Pazzano, Vincenzo
AU - Santangeli, Pasquale
AU - Baldi, Alfonso
AU - Liuzzo, Giovanna
AU - Tondo, Claudio
AU - Natale, Andrea
AU - Crea, Filippo
PY - 2011
Y1 - 2011
N2 - Aims: Histological studies support the important role of inflammation in the initiation and maintenance of atrial fibrillation (AF). We describe a novel and safe technique of atrial biopsy during AF radiofrequency catheter ablation (RFCA) to investigate the role of atrial tissue inflammation. Methods and results: We enrolled 70 consecutive patients (age 60 ± 12 years, 49 males) undergoing RFCA for AF. The control group was represented by 10 patients with WolffParkinsonWhite syndrome undergoing trans-septal puncture. Atrial biopsies were obtained by washing the dilator and needle used for trans-septal puncture with 20 mL sterile phosphate-buffered saline. The presence of intracytoplasmic C-reactive protein was assessed in formalin-fixed atrial specimens by immunohistochemistry. A sufficient amount of atrial tissue was obtained in 23/70 (32) patients with AF and in 4/10 (40) of the control group. Intracytoplasmic localization of C-reactive protein was found in isolated atrial cardiomyocytes in 11 (73) of 15 patients with paroxysmal AF as compared with 2 (25) of eight patients with persistent AF (P 0.02). Conclusions: In this study, we demonstrate the safety and feasibility of a novel technique to obtain atrial specimens during routine trans-septal puncture. Local inflammation assessed by atrial tissue localization of C-reactive protein is more likely involved in paroxysmal rather than in persistent AF
AB - Aims: Histological studies support the important role of inflammation in the initiation and maintenance of atrial fibrillation (AF). We describe a novel and safe technique of atrial biopsy during AF radiofrequency catheter ablation (RFCA) to investigate the role of atrial tissue inflammation. Methods and results: We enrolled 70 consecutive patients (age 60 ± 12 years, 49 males) undergoing RFCA for AF. The control group was represented by 10 patients with WolffParkinsonWhite syndrome undergoing trans-septal puncture. Atrial biopsies were obtained by washing the dilator and needle used for trans-septal puncture with 20 mL sterile phosphate-buffered saline. The presence of intracytoplasmic C-reactive protein was assessed in formalin-fixed atrial specimens by immunohistochemistry. A sufficient amount of atrial tissue was obtained in 23/70 (32) patients with AF and in 4/10 (40) of the control group. Intracytoplasmic localization of C-reactive protein was found in isolated atrial cardiomyocytes in 11 (73) of 15 patients with paroxysmal AF as compared with 2 (25) of eight patients with persistent AF (P 0.02). Conclusions: In this study, we demonstrate the safety and feasibility of a novel technique to obtain atrial specimens during routine trans-septal puncture. Local inflammation assessed by atrial tissue localization of C-reactive protein is more likely involved in paroxysmal rather than in persistent AF
KW - Atrial endomyocardial biopsy
KW - Atrial fibrillation
KW - Atrial endomyocardial biopsy
KW - Atrial fibrillation
UR - http://hdl.handle.net/10807/2659
U2 - 10.1093/europace/eur068
DO - 10.1093/europace/eur068
M3 - Article
SN - 1099-5129
VL - 2011
SP - 1133
EP - 1140
JO - Europace
JF - Europace
ER -