TY - JOUR
T1 - Role of flow-cytometric immunophenotyping in prediction of BCR/ABL1 gene rearrangement in adult B-cell acute lymphoblastic leukemia
AU - Corrente, Francesco
AU - Bellesi, Silvia
AU - Metafuni, Elisabetta
AU - Puggioni, Pier Luigi
AU - Marietti, Sara
AU - Ciminello, Angela Maria
AU - Za, Tommaso
AU - Sora', Federica
AU - Fianchi, Luana
AU - Sica, Simona
AU - De Stefano, Valerio
AU - Chiusolo, Patrizia
PY - 2018
Y1 - 2018
N2 - We performed a retrospective analysis of 88 adult patients with B-ALL diagnosed in our center by a flow-cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). BCR/ABL1 fusion transcripts were assessed by RT-PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1-positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1-negative cases. Moreover, BCR/ABL1-positive cases exhibited a greater median percentage and MFI values of CD13, CD33, CD66c, CD10, CD34 and CD25 expressions, but a lower median percentage and MFI values of CD38 and CD22 expressions than patients without gene rearrangement. Multivariate logistic regression analysis showed that CD10, CD38 and CD13 expressions were independent predictors for the presence of BCR/ABL1 rearrangement. Predictive probabilities of molecular occurrence based on these markers are proposed. © 2017 International Clinical Cytometry Society.
AB - We performed a retrospective analysis of 88 adult patients with B-ALL diagnosed in our center by a flow-cytometric assessment. Immunophenotypic expression of leukemic cells was explored by simultaneous evaluation of positivity, percentage of expressing cells and median fluorescence intensity (MFI). BCR/ABL1 fusion transcripts were assessed by RT-PCR analysis and were identified in 36 patients (40.9%). CD10 and CD34 were positive in the totality of BCR/ABL1-positive cases. Patients with gene rearrangement had a greater frequency of CD66c, CD13 and CD33 positivity compared with BCR/ABL1-negative cases. Moreover, BCR/ABL1-positive cases exhibited a greater median percentage and MFI values of CD13, CD33, CD66c, CD10, CD34 and CD25 expressions, but a lower median percentage and MFI values of CD38 and CD22 expressions than patients without gene rearrangement. Multivariate logistic regression analysis showed that CD10, CD38 and CD13 expressions were independent predictors for the presence of BCR/ABL1 rearrangement. Predictive probabilities of molecular occurrence based on these markers are proposed. © 2017 International Clinical Cytometry Society.
KW - 2734
KW - B cell acute lymphoblastic leukemia
KW - BCR/ABL1
KW - Cell Biology
KW - Histology
KW - immunophenotype
KW - 2734
KW - B cell acute lymphoblastic leukemia
KW - BCR/ABL1
KW - Cell Biology
KW - Histology
KW - immunophenotype
UR - http://hdl.handle.net/10807/122759
UR - http://onlinelibrary.wiley.com/journal/10.1002/(issn)1552-4957
U2 - 10.1002/cyto.b.21605
DO - 10.1002/cyto.b.21605
M3 - Article
SN - 1552-4949
VL - 94
SP - 468
EP - 476
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
ER -