Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates.

Giorgio Conti, Daniele De Luca, Angelo Minucci, Domenico Tripodi, Domenico Pietrini, Cecilia Zuppi, Ettore Domenico Capoluongo, Virgilio P Carnielli

Risultato della ricerca: Contributo in rivistaArticolo in rivista

44 Citazioni (Scopus)

Abstract

PURPOSE: Meconium aspiration syndrome (MAS) is a life-threatening neonatal lung injury, whose pathophysiology has been mainly studied in animal models. In such models, pancreatic secretory phospholipase A2 (sPLA2-IB) and proinflammatory cytokines present in meconium challenge the lungs, catabolising surfactant and harming the alveoli. Locally produced phospholipases might perpetuate the injury and influence clinical pictures and therapeutic approaches. Our aim is to verify whether pulmonary phospholipase A2 (sPLA2-IIA) is involved in the damage and to determine if phospholipases and their modulators are associated with MAS clinical pictures. METHODS: We studied distinct phospholipases A2 and their modulators in broncho-alveolar lavage (BAL) fluids and in meconium of five MAS neonates and in five control neonates ventilated for extrapulmonary reasons. RESULTS: MAS patients have higher amounts of pulmonary phospholipase (sPLA2-IIA; P = 0.016) and Clara cell secretory protein (CCSP; P = 0.032). The local production of such proteins by the lung is confirmed by their very low levels in meconium. sPLA2-IIA contributes to the higher total enzyme activity in MAS patients, as compared to controls (P = 0.008). Cytosolic phospholipase was not detected in meconium or alveolar fluid. sPLA2 activity and sPLA2-IIA concentrations are correlated with the TNFα and with the release of CCSP. sPLA2 total activity, sPLA2-IIA and TNFα concentrations in BAL fluids correlate with the oxygenation impairment and haemorrhagic lung oedema. CONCLUSIONS: Pulmonary sPLA2 is locally produced and contributes to the total sPLA2 activity during MAS. CCSP is also produced in trying to lower the inflammation. Both sPLA2 activity and sPLA2-IIA are significantly correlated with oxygenation impairment and haemorrhagic lung oedema.
Lingua originaleEnglish
pagine (da-a)1158-1165
Numero di pagine8
RivistaIntensive Care Medicine
Volume2011
Stato di pubblicazionePubblicato - 2011

Keywords

  • PLA-ICM011

Fingerprint Entra nei temi di ricerca di 'Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates.'. Insieme formano una fingerprint unica.

Cita questo