TY - JOUR
T1 - Reverse transcriptase-PCR analysis of apoptosis-regulating gene expression in human benign prostatic hyperplasia
AU - Angelucci, Cristiana
AU - Fortunata, Iacopino
AU - Gina, Lama
AU - Zelano, Giovanni
AU - Giuseppei, Gianesin
AU - Gigliola, Sica
AU - Vittorio, Bono Aldo
PY - 2005
Y1 - 2005
N2 - Background: To determine whether an imbalanced\r\ninteraction between proapototic and antiapoptotic signals may\r\naccount for the loss of the normal cell growth control in benign\r\nprostatic hyperplasia (BPH), the expression of some apoptosisregulating\r\ngenes (bcl-2, bax, c-myc, fas) was investigated. Patients\r\nand Methods: BPH specimens were obtained from 20 patients who\r\nunderwent trans-urethral resection of the prostate (TURP) or\r\nadenomectomy. Gene expression was studied by reverse\r\ntranscriptase-polymerase chain reaction (RT-PCR) and its\r\ncorrelation with age and serum PSA level was also investigated.\r\nResults: Genes were found to be differentially expressed in BPH\r\ntissues. In particular, the antiapoptotic gene bcl-2, which was\r\nfound in 18/20 samples, gave the weakest signal (p<0.05-p<0.001,\r\nWilcoxon’s signed rank test), whereas the cell cycle regulator c-myc\r\nwas detected in all the specimens and was the most highly\r\nexpressed (p<0.001). A positive relationship between the\r\nexpression of bcl-2 and that of the two proapoptotic genes bax and\r\nfas was observed (p<0.05, Spearman's rank correlation test), as\r\nwell as between c-myc and fas levels (p<0.005). Moreover, bax\r\nexpression positively correlated with age and PSA (p<0.02), which\r\nhave also been shown to directly correlate (p<0.01). Conclusion:\r\nThe higher expression of the oncogene c-myc suggests the\r\nactivation of mitogenic signals within hyperplastic prostate tissue\r\nwhich a relatively high expression of the proapoptotic genes bax\r\nand fas fails to counterbalance.
AB - Background: To determine whether an imbalanced\r\ninteraction between proapototic and antiapoptotic signals may\r\naccount for the loss of the normal cell growth control in benign\r\nprostatic hyperplasia (BPH), the expression of some apoptosisregulating\r\ngenes (bcl-2, bax, c-myc, fas) was investigated. Patients\r\nand Methods: BPH specimens were obtained from 20 patients who\r\nunderwent trans-urethral resection of the prostate (TURP) or\r\nadenomectomy. Gene expression was studied by reverse\r\ntranscriptase-polymerase chain reaction (RT-PCR) and its\r\ncorrelation with age and serum PSA level was also investigated.\r\nResults: Genes were found to be differentially expressed in BPH\r\ntissues. In particular, the antiapoptotic gene bcl-2, which was\r\nfound in 18/20 samples, gave the weakest signal (p<0.05-p<0.001,\r\nWilcoxon’s signed rank test), whereas the cell cycle regulator c-myc\r\nwas detected in all the specimens and was the most highly\r\nexpressed (p<0.001). A positive relationship between the\r\nexpression of bcl-2 and that of the two proapoptotic genes bax and\r\nfas was observed (p<0.05, Spearman's rank correlation test), as\r\nwell as between c-myc and fas levels (p<0.005). Moreover, bax\r\nexpression positively correlated with age and PSA (p<0.02), which\r\nhave also been shown to directly correlate (p<0.01). Conclusion:\r\nThe higher expression of the oncogene c-myc suggests the\r\nactivation of mitogenic signals within hyperplastic prostate tissue\r\nwhich a relatively high expression of the proapoptotic genes bax\r\nand fas fails to counterbalance.
KW - Benign prostatic hyperplasia
KW - apoptosis
KW - bax
KW - bcl-2
KW - c-myc
KW - fas
KW - Benign prostatic hyperplasia
KW - apoptosis
KW - bax
KW - bcl-2
KW - c-myc
KW - fas
UR - https://publicatt.unicatt.it/handle/10807/160813
UR - https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=27944448370&origin=inward
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=27944448370&origin=inward
M3 - Article
SN - 0250-7005
VL - 25
SP - 3937
EP - 3941
JO - Anticancer Research
JF - Anticancer Research
IS - 6B
ER -