Relationship among expression, amplification, and methylation of FE4 esterase genes in Italian populations of Myzus persicae (Sulzer) (Homoptera: Aphididae).

Emanuele Mazzoni, Ferdinando Pavesi, Piero Cravedi, D. Bizzaro, E. Barbolini, S. Giannini, S. Cassanelli, G. C. Manicardi

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15 Citazioni (Scopus)

Abstract

The wide use of insecticides containing an esteric group selected resistant Myzus persicae populations characterised by the overproduction of one of two closely related carboxylesterases (E4 and FE4). In this paper, we present data collected from Italian population indicating that all the 22 populations analysed possess amplified FE4 gene only. The estimation of FE4 copy number, carried out by densitometric scanning of dot and Southern blots, puts in evidence that the different populations possess a gene copy number ranging from 6 to 104. Statistical analysis shows the existence of a high positive correlation between gene copy number and total esterase activity. In aphid strain with low FE4 copy number, these genes are almost totally methylated. On the contrary, aphid strains with high FE4 gene number evidenced highly variable methylation levels and absence of correlation between the number of genes and their methylation state. The same result has been observed when comparing FE4 methylation levels and esterase activity
Lingua originaleEnglish
pagine (da-a)51-58
Numero di pagine8
RivistaPesticide Biochemistry and Physiology
Volume81
DOI
Stato di pubblicazionePubblicato - 2005

Keywords

  • Esterase
  • Gene amplification
  • Insecticide resistance
  • Methylation
  • Myzus persicae

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